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Literature summary for 1.14.14.94 extracted from

  • Bardowell, S.A.; Stec, D.E.; Parker, R.S.
    Common variants of cytochrome P450 4F2 exhibit altered vitamin E-omega-hydroxylase specific activity (2010), J. Nutr., 140, 1901-1906.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.94

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and CYP4F2 mutant enzymes in Spodoptera frugiperda SF9 insect cell microsomes via the baculovrius transfection system Homo sapiens

Protein Variants

Protein Variants Comment Organism
V433M naturally occuring common genetic variant of the human CYP4F2 gene, the mutant has lower enzyme specific activity toward tocopherols, 42-66% of wild-type activity, but is without a significant effect on the metabolism of tocotrienols Homo sapiens
W12G naturally occuring common genetic variant of the human CYP4F2 gene, the mutant exhibits a greater enzyme specific activity compared to the wild-type enzyme for both tocopherols and tocotrienols, 230-275% of wild-type activity towards alpha, gamma, and delta-tocopherol and 350% of wild-type activity towards alpha, gamma, and delta-tocotrienol Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, recombinant wild-type an dmutant enzymes, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozyme CYP4F2
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-tocopherol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens alpha-13'-hydroxy-tocopherol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
alpha-tocotrienol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens ?
-
 ?
delta-tocopherol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens delta-13'-hydroxy-tocopherol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
delta-tocotrienol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens ?
-
 ?
gamma-tocopherol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 13'-hydroxy-gamma-tocopherol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
gamma-tocotrienol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, recombinant wild-type an dmutant enzymes, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CYP4F2
-
 Homo sapiens
cytochrome P450 4F2
-
 Homo sapiens
TOH-omega-hydroxylase
-
 Homo sapiens
vitamin E-omega-hydroxylase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism CYP4F catalyzes the omega-hydroxylation of the side chain of tocopherols and tocotrienols, the first step in their catabolism to polar metabolites excreted in urine. CYP4F2 is the only enzyme currently shown to metabolize vitamin E in humans Homo sapiens