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Literature summary for 1.14.14.94 extracted from
- Prostaglandin and leukotriene omega-hydroxylases (2002), Prostaglandins, 68-69, 345-362.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in yeast | Homo sapiens |
| expression in yeast cells, CYP4F3 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00064 | - |
leukotriene B4 | CYP4F3 | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the isolated cDNA encodes a protein of 520 amino acids with 59805 Da, CYP4F3 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | the enzyme is the principal arachidonate omega-hydroxylase in liver, CYP4F2 | 20-hydroxy-5,8,11,14-eicosatetraenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | the enzyme plays a critical role in regulating the biological effect of leukotriene B4, CYP4F3 | 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | Homo sapiens | CYP4F3B and CYP4F2 may function collaboratively as 20-HETE synthetase as well as omega-hydroxylases for leukotiene b4 and other lipoxygenase-dependent eicosanoids in human liver and other tissues | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CYP4F3 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | CYP4F11 | Homo sapiens | - |
| ileum | CYP4F3B is the predominant CYP4F isoform | Homo sapiens | - |
| kidney | CYP4F2 | Homo sapiens | - |
| kidney | CYP4F11 | Homo sapiens | - |
| kidney | CYP4F3B | Homo sapiens | - |
| liver | CYP4F2 | Homo sapiens | - |
| liver | CYP4F11 | Homo sapiens | - |
| liver | CYP4F3B is the predominant CYP4F isoform | Homo sapiens | - |
| liver | mRNA for CYP4F8 detected | Homo sapiens | - |
| additional information | CYP4F2 is not expressed in myeloid cells, polymorphonuclear leukocyte | Homo sapiens | - |
| additional information | no expression of CYP4F8 in leukocytes | Homo sapiens | - |
| polymorphonuclear leukocyte | CYP4F3 is specifically expressed in. CYP4F3 is restricted to myeloid cells | Homo sapiens | - |
| prostate gland | mRNA for CYP4F8 detected | Homo sapiens | - |
| seminal vesicle | CYP4F8 | Homo sapiens | - |
| skeletal muscle | CYP4F11 | Homo sapiens | - |
| skin | CYP4F2 | Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 50% losss of activity after 3 days | Homo sapiens |
| -70°C, no loss of activity with highly purified preparations even after 4 years | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,Z,Z,Z)-8-hydroxy-5,9,11,14-eicosatetraenoic acid + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F2 | Homo sapiens | ? | - |
? | |
| 12-hydroxy-5,8,10,14-eicosatetraenoic acid + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F2 | Homo sapiens | ? | - |
? | |
| 20-hydroxy-leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F3 | Homo sapiens | ? | - |
? | |
| 5-hydroxy-6,8,11,14-eicosatetraenoic acid + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F3 | Homo sapiens | ? | - |
? | |
| 6-trans-leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F2 | Homo sapiens | ? | - |
? | |
| 6-trans-leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F3 | Homo sapiens | ? | - |
? | |
| 9,11-diazo-15-deoxy-prostaglandin H2 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F8 | Homo sapiens | ? | - |
? | |
| 9,11-epoxymethano-prostaglandin H2 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F8 | Homo sapiens | ? | - |
? | |
| arachidonate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 20-hydroxy-5,8,11,14-eicosatetraenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| arachidonate + [reduced NADPH-hemoprotein reductase] + O2 | very poor substrate | Homo sapiens | 20-hydroxy-5,8,11,14-eicosatetraenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| arachidonate + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is the principal arachidonate omega-hydroxylase in liver, CYP4F2 | Homo sapiens | 20-hydroxy-5,8,11,14-eicosatetraenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 18(R)-hydroxyarachidonate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme plays a critical role in regulating the biological effect of leukotriene B4, CYP4F3 | Homo sapiens | 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 | yielding omega-COOH-leukotriene B4 via omega-hydroxy-leukotriene B4 and omega-CHO-leukotriene B4, CYP4F3 | Homo sapiens | 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lipoxin A4 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F3 preferentially omega-jydroxylates lipoxin B4 over lipoxin A4 | Homo sapiens | ? | - |
? | |
| lipoxin A4 + [reduced NADPH-hemoprotein reductase] + O2 | no omega-hydroxylation of LXB4, CYP4F2 | Homo sapiens | ? | - |
? | |
| lipoxin B4 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F3 preferentially omega-jydroxylates lipoxin B4 over lipoxin A4 | Homo sapiens | ? | - |
? | |
| additional information | CYP4F3B and CYP4F2 may function collaboratively as 20-HETE synthetase as well as omega-hydroxylases for leukotiene b4 and other lipoxygenase-dependent eicosanoids in human liver and other tissues | Homo sapiens | ? | - |
? | |
| prostaglandin H1 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F8 | Homo sapiens | 19-hydroxy-prostaglandin H1 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| prostaglandin H2 + [reduced NADPH-hemoprotein reductase] + O2 | CYP4F8 | Homo sapiens | 19-hydroxy-prostaglandin H2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP4F11 | - |
Homo sapiens |
| CYP4F2 | - |
Homo sapiens |
| CYP4F3 | - |
Homo sapiens |
| CYP4F3B | - |
Homo sapiens |
| CYP4F8 | - |
Homo sapiens |
| LTB4 omega hydroxylase | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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