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Literature summary for 1.14.14.94 extracted from

  • Romano, M.C.; Eckardt, R.D.; Bender, P.E.; Leonard, T.B.; Straub, K.M.; Newton, J.F.
    Biochemical characterization of hepatic microsomal leukotriene B4 hydroxylases (1987), J. Biol. Chem., 262, 1590-1595.
    View publication on PubMed
Search for more literature for 1.14.14.94

Activating Compound

Activating Compound Comment Organism Structure
additional information hepatic enzyme is not induced by phenobarbital or 3-methylcholanthrene Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
alpha-naphthoflavone
-
 Rattus norvegicus
Metyrapone
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 leukotriene B4
-
 Rattus norvegicus
0.0661
-
 leukotriene B4 cosubstrate NADPH Rattus norvegicus
0.868
-
 leukotriene B4 cosubstrate NADH Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-
additional information not cytosol Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus inactivation of leukotriene B4, a mediator of inflammation (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 inactivation of leukotriene B4, a mediator of inflammation Rattus norvegicus (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate Rattus norvegicus 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 completely inactivated after 1 h Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 enzyme contains cytochrome P-450 Rattus norvegicus