Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.93 extracted from

  • Schopfer, C.R.; Kochs, G.; Lottspeich, F.; Ebel, J.
    Molecular characterization and functional expression of dihydroxypterocarpan 6a-hydroxylase, an enzyme specific for pterocarpanoid phytoalexin biosynthesis in soybean (Glycine max L.) (1998), FEBS Lett., 432, 182-186.
    View publication on PubMed
Search for more literature for 1.14.14.93

Cloned(Commentary)

Cloned (Comment) Organism
CYP93A1 cDNA isolated from elicitor-induced soybean cells encodes the enzyme, CYP93A1 expressed in Saccharomyces cerevisiae Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 (6aR,11aR)-3,9-dihydroxypterocarpan recombinant enzyme expressed in yeast Glycine max
0.00016
-
 (6aR,11aR)-3,9-dihydroxypterocarpan enzyme from elicitor-treated soybean cells Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Glycine max
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 Glycine max the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycine max (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean Glycine max (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme and soybean microsomes Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 recombinant enzyme and soybean microsomes Glycine max