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Literature summary for 1.14.14.92 extracted from

  • Kim, D.; Kim, S.W.; Choi, K.Y.; Lee, J.S.; Kim, E.
    Molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chromohalobacter sp. strain HS-2 (2008), FEMS Microbiol. Lett., 280, 235-241.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
PCR amplification, expression in Escherichia coli BL21 (DE3) Chromohalobacter sp. HS-2

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 10% is optimal NaCl concentration for Chromohalobacter sp. strain HS-2 Chromohalobacter sp. HS-2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoate + [reduced NADPH-hemoprotein reductase] + O2 Chromohalobacter sp. HS-2
-
 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?
benzoate + [reduced NADPH-hemoprotein reductase] + O2 Chromohalobacter sp. HS2
-
 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?

Organism

Organism UniProt Comment Textmining
Chromohalobacter sp. HS-2 A8I4E8 ben A subunits; isolated from salted fermented clams
-
Chromohalobacter sp. HS-2 A8I4F1 ben B subunits; isolated from salted fermented clams
-
Chromohalobacter sp. HS-2 A8I4F4 ben C subunits; isolated from salted fermented clams
-
Chromohalobacter sp. HS2 A8I4E8 ben A subunits; isolated from salted fermented clams
-
Chromohalobacter sp. HS2 A8I4F1 ben B subunits; isolated from salted fermented clams
-
Chromohalobacter sp. HS2 A8I4F4 ben C subunits; isolated from salted fermented clams
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Chromohalobacter sp. HS-2 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Chromohalobacter sp. HS2 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further ?

Synonyms

Synonyms Comment Organism
benzoate 1,2-dioxygenase product of genes benA (large subunit), benB (small subunit), benC (electron transfer component) Chromohalobacter sp. HS-2