Cloned (Comment) | Organism |
---|---|
gene CYP53B1, functional overexpression in Yarrowia lipolytica strain UOFS Y-2366, multiple copies of CYP53B1 cDNA cloned under control of POX2 promoter, construction of several clones, feeding of stearic acid to the recombinant yeast leads to high accumulation of 4-hydroxybenzoate | Cystobasidium minutum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | Cystobasidium minutum | - |
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | Cystobasidium minutum CBS 2177 | - |
4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cystobasidium minutum | - |
gene CYP53B1 | - |
Cystobasidium minutum CBS 2177 | - |
gene CYP53B1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme expressed in Yarrowia lipolytica clones, overview | Cystobasidium minutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cystobasidium minutum | 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cystobasidium minutum CBS 2177 | 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
benzoate para-hydroxylase | - |
Cystobasidium minutum |
CYP53B1 | - |
Cystobasidium minutum |