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Literature summary for 1.14.14.92 extracted from
- Purification, properties and induction of a specific benzoate-4-hydroxylase from Aspergillus niger (UBC 814) (1975), Biochim. Biophys. Acta, 384, 46-57.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | 30% of activity with NADPH as electron donor | Aspergillus niger |  |
General Stability
| General Stability | Organism |
|---|---|
| benzoate stabilizes | Aspergillus niger |
| EDTA stabilizes | Aspergillus niger |
| glutathione stabilizes | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 0.05 mM; 50% inhibition | Aspergillus niger | |
| 8-hydroxyquinoline | 5 mM, complete inhibition | Aspergillus niger | |
| Cu2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
| diethyldithiocarbamate | 5 mM, 95% inhibition | Aspergillus niger | |
| Hg2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
| Mg2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
| Mn2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
| Mo2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
| N-ethylmaleimide | - |
Aspergillus niger | |
| o-phenanthroline | 0.05 mM, 70% inhibition | Aspergillus niger | |
| oxalate | 2 mM, 20% inhibition | Aspergillus niger | |
| p-chloromercuribenzoate | - |
Aspergillus niger | |
| Zn2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
benzoate | - |
Aspergillus niger | |
| 0.16 | - |
NADPH | - |
Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for activity | Aspergillus niger | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger UBC 814 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, tricalcium phosphate gel, DEAE-cellulose, alumina C-gamma gel | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0888 | - |
- |
Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, 40% loss of activity | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoate + NADPH + H+ + O2 | - |
Aspergillus niger | 4-hydroxybenzoate + NADP+ + H2O | - |
r | |
| benzoate + NADPH + H+ + O2 | - |
Aspergillus niger UBC 814 | 4-hydroxybenzoate + NADP+ + H2O | - |
r | |
| benzoate + [reduced NADPH-hemoprotein reductase] + O2 | specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor | Aspergillus niger | 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| benzoate + [reduced NADPH-hemoprotein reductase] + O2 | specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor | Aspergillus niger UBC 814 | 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 38 | rapid decrease of activity above 38°C and below 25°C | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | approx. 35% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 8.0 | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydropteridine | required as prosthetic group, maximal activity with 6,7-dimethyl 5,6,7,8-tetrahydropteridine, 60% activity with biopterine and tetrahydrofolic acid | Aspergillus niger | |
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