Literature summary for 1.14.14.87 extracted from

Pandey, A.; Misra, P.; Khan, M.; Swarnkar, G.; Tewari, M.; Bhambhani, S.; Trivedi, R.; Chattopadhyay, N.; Trivedi, P.
Co-expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity (2014), Plant Biotechnol. J., 12, 69-80 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis	Glycine max

Cloned (Comment)

Organism

gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis

Glycine max

Protein Variants

Protein Variants	Comment	Organism
additional information	oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols	Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2	Glycine max	-	2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Glycine max	Q9M6D6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2	-	Glycine max	2,4',7-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
GmIFS1	-	Glycine max
isoflavone synthase	-	Glycine max

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Glycine max

Expression

Organism	Comment	Expression
Glycine max	gene ifs1, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana benthamiana leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity	up