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Literature summary for 1.14.14.87 extracted from

  • Sawada, Y.; Kinoshita, K.; Akashi, T.; Aoki, T.; Ayabe, S.
    Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase (2002), Plant J., 31, 555-564.
    View publication on PubMed
Search for more literature for 1.14.14.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BJ2168 Glycyrrhiza echinata

Protein Variants

Protein Variants Comment Organism
K375T the mutant catalyses the aryl migration from liquiritigenin to yield 3,7,4'-trihydroxyflavanone only, its pH optimum is at pH 6.5 Glycyrrhiza echinata
S310T the mutant also catalyses the aryl migration from liquiritigenin to yield 2,7,4'-trihydroxyisoflavanone, but the ratio of the by-product (3,7,4'-trihydroxyflavanone) formation is increased from 8% to 36% (pH 7.5), and a small amount (7%) of a new product, 7,4'-dihydroxyflavone, is detected compared to the wild type Glycyrrhiza echinata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 7,4'-dihydroxyflavanone mutant enzyme S310T, at pH 7.5, 30°C Glycyrrhiza echinata
0.008
-
 7,4'-dihydroxyflavanone wild type enzyme, at pH 7.5, 30°C Glycyrrhiza echinata
0.011
-
 7,4'-dihydroxyflavanone mutant enzyme K375T, at pH 7.5, 30°C Glycyrrhiza echinata

Organism

Organism UniProt Comment Textmining
Glycyrrhiza echinata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,4'-dihydroxyflavanone + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 i.e. liquirtigenin Glycyrrhiza echinata 2,7,4'-trihydroxyisoflavanone + 3,7,4'-trihydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O 3,7,4'-trihydroxyflavanone is the by-product of the reaction (8% yield) ?

Synonyms

Synonyms Comment Organism
2-hydroxyisoflavanone synthase
-
 Glycyrrhiza echinata
CYP93C2
-
 Glycyrrhiza echinata
IFS
-
 Glycyrrhiza echinata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Glycyrrhiza echinata