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Literature summary for 1.14.14.86 extracted from

  • Okazaki, M.; Nimitkeatkai, H.; Muramatsu, T.; Aoyama, H.; Ueno, K.; Mizutani, M.; Hirai, N.; Kondo, S.; Ohnishi, T.; Todoroki, Y.
    Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8-hydroxylase CYP707A (2011), Bioorg. Med. Chem., 19, 406-413.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
CYP701A, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate i.e. abscinazole-E1, orUT1-E2Ts, or Abz-E1, a specific potent inhibitor of ABA 8'-hydroxylase, EC 1.14.13.93, but a weak inhibitor of CYP701A, both in vitro and in vivo Oryza sativa
abscinazole-F1
-
Oryza sativa
DSI-505ME
-
Oryza sativa
uniconazole i.e. UNI Oryza sativa
UT4
-
Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Oryza sativa
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oryza sativa CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin ?
-
?
additional information Oryza sativa Nipponbare CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin ?
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-
Oryza sativa Nipponbare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CYP701A partially from insect Sf9 cells in microsomes Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
Oryza sativa ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
Oryza sativa Nipponbare ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
additional information CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin Oryza sativa ?
-
?
additional information CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin Oryza sativa Nipponbare ?
-
?

Synonyms

Synonyms Comment Organism
CYP701A
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.25
-
assay at Oryza sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00097
-
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate pH 7.25, 30°C, recombinant enzyme Oryza sativa