Any feedback?
Literature summary for 1.14.14.84 extracted from
- Protein components of a cytochrome P-450 linalool 8-methyl hydroxylase (1990), J. Biol. Chem., 265, 1345-1351.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CO | complete inhibition; complete inhibition of the oxidation reactions | Pseudomonas putida |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | requirement of two enzyme components, iron-heme protein and iron-sulfur protein | Pseudomonas putida | |
| Fe2+ | heme-containing cytochrome P450 enzyme | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
multi-component enzyme consisting of LIN-reductase, Fe2-S2-redoxin and cytochrome LIN-P450. MW of the reductase: 43700 Da, analytical data from amino acid composition and prosthetic group quantification, 45000 Da, gel filtration. MW of Fe2-S2-redoxin: 11000 Da, gel filtration, 12800 Da, analytical data from amino acid composition and prosthetic group quantification. MW of cytochrome LIN-P450: 44800 Da, analytical data from amino acid composition and prosthetic group quantification, 45000 Da, gel filtration | Pseudomonas putida |
| 43700 | - |
1 * 44000, SDS-PAGE, 1 * 43700, about, sequence calculation | Pseudomonas putida |
| 44000 | - |
1 * 44000, SDS-PAGE, 1 * 43700, about, sequence calculation | Pseudomonas putida |
| 45000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | Pseudomonas putida | linalool, initial reaction in linalool catabolism | (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | Pseudomonas putida PpG777 | linalool, initial reaction in linalool catabolism | (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Pseudomonas putida | - |
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
| linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Pseudomonas putida PpG777 | - |
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida | - |
i.e. Pseudomonas incognita | - |
| Pseudomonas putida PpG777 | - |
i.e. Pseudomonas incognita | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by anion exchange chromatography, ammonium sulfate fractionation, a second step of anion exchange chromatography, affinity chromatography, a thrid step of anion exchange chromatography, and two further different steps of affinity chromatography to over 90% hommogeneity | Pseudomonas putida |
| purification of 3 enzyme components: iron-sulphur, FAD and P-450 | Pseudomonas putida |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 = (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | mixed function monooxygenase consisting of LIN-reductase, Fe2S2-redoxin and cytochrome LIN P-450 | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain PpG777 utilizes linalool as sole carbon and energy source | Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0042 | - |
reductase | Pseudomonas putida |
| 0.014 | - |
purified enzyme, pH 7.4, temperature not specified in the publication | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -196°C, LIN-redoxin reductase and cytochrome LINP-450 in solution stable over a long period after ultrafiltration or dialysis and repeated freeze/thaw-cycles | Pseudomonas putida |
| 0°C, LIN-redoxin loses its prosthetic group, 5 mM DTT retards apoprotein formation | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6E)-8-hydroxylinalool + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pseudomonas putida | (6E)-8-oxolinalool + [oxidized NADPH-hemoprotein reductase] + H2O | product analysis by mass spectrometry | ? | |
| (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [reduced NADPH-hemoprotein reductase] + O2 | 8-hydroxylinalool | Pseudomonas putida | 8-oxolinalool + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [reduced NADPH-hemoprotein reductase] + O2 | 8-hydroxylinalool | Pseudomonas putida PpG777 | 8-oxolinalool + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pseudomonas putida | (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O | 8-hydroxylinalool | ? | |
| 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | linalool, initial reaction in linalool catabolism | Pseudomonas putida | (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pseudomonas putida PpG777 | (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O | 8-hydroxylinalool | ? | |
| 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 | linalool, initial reaction in linalool catabolism | Pseudomonas putida PpG777 | (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Pseudomonas putida | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
| linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | overall reaction | Pseudomonas putida | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | product analysis by mass spectrometry | ? | |
| linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Pseudomonas putida PpG777 | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
| linalool + NADH + O2 | - |
Pseudomonas putida | (6E)-8-hydroxylinalool + NAD+ + H2O | - |
r | |
| linalool + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pseudomonas putida | (6E)-8-hydroxylinalool + [oxidized NADPH-hemoprotein reductase] + H2O | product analysis by mass spectrometry | ? | |
| additional information | the enzyme does not perform 10-methyl hydroxylation of linalool | Pseudomonas putida | ? | - |
? | |
| additional information | the enzyme does not perform 10-methyl hydroxylation of linalool | Pseudomonas putida PpG777 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE, 1 * 43700, about, sequence calculation | Pseudomonas putida |
| More | enzyme consists of LIN-reductase, MW 44000 Da, and Fe2S2-redoxin, MW 10700 Da, and cytochrome LIN-P450, MW 47000 Da, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome P-450 linalool 8-hydroxylase | - |
Pseudomonas putida |
| cytochrome P-450-dependent linalool 8-hydroxylase | - |
Pseudomonas putida |
| LIN reductase | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
NADH | substrate-stimulated NADH oxidation using excess amounts of P-450 and FAD component of the enzyme | Pseudomonas putida | |
| 32 | - |
NADH | substrate-stimulated NADH oxidation using excess amounts of iron-sulfur and FAD component of the enzyme | Pseudomonas putida | |
| 43 | - |
NADH | substrate-stimulated NADH oxidation using excess amounts of iron sulfur and P-450 component of the enzyme | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Pseudomonas putida | |
| heme | - |
Pseudomonas putida | |
| redoxin | an Fe2/S2/Cys4 protein | Pseudomonas putida |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas putida | sequence calculation | - |
4.65 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme catalyzes the first step in linalool catabolism | Pseudomonas putida |
| additional information | analysis of the heme-thiolate reaction center, molecular domains of substrate specificity, redox-pair interactions, and the regulation of the reaction cycle, overview | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
