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Literature summary for 1.14.14.83 extracted from

  • Wang, J.; Liu, Y.; Cai, Y.; Zhang, F.; Xia, G.; Xiang, F.
    Cloning and functional analysis of geraniol 10-hydroxylase, a cytochrome P450 from Swertia mussotii Franch (2010), Biosci. Biotechnol. Biochem., 74, 1583-1590.
    View publication on PubMed
Search for more literature for 1.14.14.83

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP76B10, DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression analysis, sequence comparison and phylogenetic tree, expression in Pichia pastoris and in Escherichia coli Swertia mussotii
SmG10H, DNA and amno acid sequence determination and analysis, sequence comparisons and phylogenetic tree, semiquantitative RT-PCR expression analysis, recombinant expression in Escherichia coli strain BL21 and in Pichia pastoris Swertia mussotii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Swertia mussotii 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55500
-
 x * 55500, about, sequence calculation Swertia mussotii
55500
-
 x * 55500, recombinant G10H, SDS-PAGE Swertia mussotii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2 Swertia mussotii
-
 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Swertia mussotii CYP76B10 is involved in production of swertiamarin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Swertia mussotii D1MI46
-
-
Swertia mussotii D1MI46 gene CYP76B10
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression level Swertia mussotii
-
root
-
 Swertia mussotii
-
stem
-
 Swertia mussotii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Swertia mussotii 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Swertia mussotii 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O mass spectrometrical product analysis ?
additional information CYP76B10 is involved in production of swertiamarin Swertia mussotii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55500, about, sequence calculation Swertia mussotii
? x * 55500, recombinant G10H, SDS-PAGE Swertia mussotii

Synonyms

Synonyms Comment Organism
CYP76B10
-
 Swertia mussotii
CYP76B10 the enzyme belongs to the CYP76 family of enzymes Swertia mussotii
G10H
-
 Swertia mussotii

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Swertia mussotii

Expression

Organism Comment Expression
Swertia mussotii G10H expression, recombinant in Escherichia coli, is upregulated by methyljasmonate 6-36 h after treatment up
Swertia mussotii methyljasmonate induces CYP76B10 expression up

General Information

General Information Comment Organism
metabolism G10H plays a role in the iridoid monoterpene and indole alkaloid biosynthesis Swertia mussotii
metabolism the enzyme plays an important role in the iridoid monoterpenoid and the indole alkaloid biosynthesis, overview Swertia mussotii
additional information geraniol 10-hydroxylase is a cytochrome P450 monooxygenase Swertia mussotii
physiological function G10H hydroxylates geraniol and is involved in biosynthesis of swertiamarin via the iridoid pathway and of strictosidine via the indole pathway, overview, induced by methyljasmonate Swertia mussotii