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Literature summary for 1.14.14.82 extracted from

  • Park, S.; Choi, M.J.; Lee, J.Y.; Kim, J.K.; Ha, S.H.; Lim, S.H.
    Molecular and biochemical analysis of two rice flavonoid 3-hydroxylase to evaluate their roles in flavonoid biosynthesis in rice grain (2016), Int. J. Mol. Sci., 17, 1549 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in yeast strain WAT11 Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000072
-
apigenin pH 7.5, 30°C, enzyme from white rice Oryza sativa Japonica Group
0.000085
-
apigenin pH 7.5, 30°C, enzyme from black rice Oryza sativa Japonica Group
0.000098
-
kaempferol pH 7.5, 30°C, enzyme from black rice Oryza sativa Japonica Group
0.000108
-
naringenin pH 7.5, 30°C, enzyme from black rice Oryza sativa Japonica Group
0.00011
-
kaempferol pH 7.5, 30°C, enzyme from white rice Oryza sativa Japonica Group
0.00024
-
apigenin pH 7.5, 30°C, enzyme from white rice Oryza sativa Japonica Group
0.000286
-
naringenin pH 7.5, 30°C, enzyme from white rice Oryza sativa Japonica Group
0.00103
-
dihydrokaempferol pH 7.5, 30°C, enzyme from black rice Oryza sativa Japonica Group
0.002494
-
dihydrokaempferol pH 7.5, 30°C, enzyme from white rice Oryza sativa Japonica Group
0.0029
-
kaempferol pH 7.5, 30°C, enzyme from white rice Oryza sativa Japonica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q7G602 cDNA clones of CYP75B3 are isolated from the white rice, Iimi (IM), black rice, Heugnam (HN) and Heugjinju (HJJ), and red rice, Jeogjinju (JJJ) and Hongjinju (HoJJ). Three cDNA clones of CYP75B3 encoded proteins of 526 amino acids (CYP75B3-IM, CYP75B3-HN, and CYP75B3-JJJ). Amino acid sequence comparison reveals that CYP75B3-IM and CYP75B3-JJJ are identical to the registered sequence in UNIPROT database, but one amino acid is substituted in the CYP75B3-HN sequence at position 27. An additional substitution is observed in the CYP75B4-HJJ sequence at position 258. His27 of CYP75B3-HN is located at the N-terminal membrane anchor region; however, the other cytochrome P450-specific conserved regions, such as the oxygen binding pocket, the ExxR motif, and the heme binding domain, are identical among all of the isolated genes, while most of the substrate recognition sites are different between CYP75B3s. The Thr504 of each CYP75B3, known as functional determinant for the specific enzyme activity, is conserved
-
Oryza sativa Japonica Group Q8LM92 cDNA clones of CYP75B4 are isolated from the white rice, Iimi (IM), black rice, Heugnam (HN) and Heugjinju (HJJ), and red rice, Jeogjinju (JJJ) and Hongjinju (HoJJ). Five cDNA clones of CYP75B4-encoded proteins of 535 amino acids (CYP75B4-IM, CYP75B4-HN, CYP75B4-HJJ, CYP75B4-JJJ, and CYP75B4-HoJJ). Amino acid sequence comparison reveal that CYP75B4-IM is identical to the registered sequence in UNIPROT, but an amino acid substitution at position 351 is shared by CYP75B4-HN and CYP75B4-HJJ from black rice and another substitution at positions seven is shared by CYP75B4-JJJ and CYP75B4-HoJJ from red rice. An additional substitution is observed in the CYP75B4-HJJ sequence at position 258. Val7 of CYP75B4-JJJ and CYP75B4-HoJJ are located at the N-terminal membrane anchor region; however, the other cytochrome P450-specific conserved regions, such as the oxygen binding pocket, the ExxR motif, and the heme binding domain, are identical among all of the isolated genes, while most of the substrate recognition sites are different between CYP75B4s. The Leu512 of each CYP75B4, known as functional determinant for the specific enzyme activity, is conserved. The other substitutions, His258 of CYP75B4-HJJ and Gln351 of CYP75B4-HN and CYP75B4-HJJ, are located out of the functional regions
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf highly expressed Oryza sativa Japonica Group
-
root weakly expressed Oryza sativa Japonica Group
-
seed expressed predominantly in the developing seeds of black rice and not in those of white and red rice Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa Japonica Group luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
apigenin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme exhibits a higher preference for apigenin than for other substrates Oryza sativa Japonica Group luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 the enzyme has a low preference of dihydrokaempferol Oryza sativa Japonica Group dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa Japonica Group quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2 the enzyme prefers kaempferol to other substrates Oryza sativa Japonica Group quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa Japonica Group eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP75B3
-
Oryza sativa Japonica Group
CYP75B4
-
Oryza sativa Japonica Group
flavonoid 3'-hydroxylase
-
Oryza sativa Japonica Group

General Information

General Information Comment Organism
metabolism the enzyme is involved in flavonoid pathway Oryza sativa Japonica Group