Any feedback?
Literature summary for 1.14.14.82 extracted from
- Identification of the molecular basis for the functional difference between flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase (2007), FEBS Lett., 581, 3429-3434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes | Gerbera hybrid cultivar |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of chimeric constructs consisting of cDNA fragments of Gerbera hybrida F3'H and Osteospermum hybrida F3'5'H, EC 1.14.13.88, overview | Gerbera hybrid cultivar |
| T484F | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Gerbera hybrid cultivar |
| T487A | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Gerbera hybrid cultivar |
| T487S | site-directed mutagenesis, a conservative Thr to Ser exchange at position 487 conferred additional 5'-hydroxylation activity to recombinant Gerbera hybrida F3'H | Gerbera hybrid cultivar |
| Y484F/T487S | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Gerbera hybrid cultivar |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | a cytochrome P450 enzyme | Gerbera hybrid cultivar |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | Gerbera hybrid cultivar | - |
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | Gerbera hybrid cultivar | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | ? | - |
? | |
| naringenin + [reduced NADPH-hemoprotein reductase] + O2 | Gerbera hybrid cultivar | - |
eriodyctiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gerbera hybrid cultivar | Q38L00 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities of recombinant chimeric mutant enzymes, overview | Gerbera hybrid cultivar |
| 0.000003 | - |
recombinant wild-type F3'H | Gerbera hybrid cultivar |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Gerbera hybrid cultivar | dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | substrate specificity of F3'H and the F3'5'H, flavonoid 3',5'-hydroxylase, EC 1.14.13.88, evolutionary derived from F3'H, is determined near the N-terminal end and the functional difference between these two enzymes near the C-terminal end, relatively few amino acids exchanges are required for the evolutionary extension of 3'- to 3',5'-hydroxylation activity, overview | Gerbera hybrid cultivar | ? | - |
? | |
| naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Gerbera hybrid cultivar | eriodyctiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| naringenin + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate of the wild-type enzyme | Gerbera hybrid cultivar | eriodyctiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F3'H | - |
Gerbera hybrid cultivar |
| flavonoid 3'-hydroxylase | - |
Gerbera hybrid cultivar |
| More | the enzyme belongs to the subfamilies CYP75B of the superfamily of cytochrome P450-dependent monooxygenases | Gerbera hybrid cultivar |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Gerbera hybrid cultivar |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Gerbera hybrid cultivar |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome | a cytochrome P450 enzyme | Gerbera hybrid cultivar |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
