General Stability | Organism |
---|---|
sucrose or glycerol plus dithiothreitol stabilize during purification and storage at -70°C | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha'-dipyridyl | partial | Zea mays | |
CO | inhibition reduced by irradiation with 450 nm light during incubation | Zea mays | |
cytochrome c | - |
Zea mays | |
FeCl3 | - |
Zea mays | |
N-ethylmaleimide | - |
Zea mays | |
NADP+ | - |
Zea mays | |
NaN3 | - |
Zea mays | |
p-chloromercuribenzoate | partial | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Zea mays | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Zea mays | - |
Storage Stability | Organism |
---|---|
-70°C, sucrose or glycerol plus dithiothreitol stabilize during purification and storage | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apigenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | luteolin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | no activity | Zea mays | dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
kaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | quercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
10 min, activity destroyed | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
sharp drop in activity on either side of the optimum of pH 8.5 | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | 0.21 nmol per mg of protein | Zea mays |