Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | increases the enzyme activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene CYP4A11, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K276T | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
S353G | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
W126R | site-directed mutagenesis, the mutant W126R variant cannot be expressed in Escherichia coli. | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, fatty acids binding constants | Homo sapiens | |
0.014 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
0.018 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
0.018 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
0.019 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.021 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
0.021 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
0.021 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 | Homo sapiens | |
0.022 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.022 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
0.023 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
0.026 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.029 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
0.03 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
0.033 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
0.033 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
0.037 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
0.037 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
0.045 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in heme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q02928 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58500, recombinant His-tagged wild-type enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
omega-hydroxylase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.068 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
0.088 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
0.1 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
0.115 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
0.117 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.157 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.188 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
0.193 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
0.258 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
0.258 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 | Homo sapiens | |
0.278 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
0.423 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
0.5 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
0.502 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
0.822 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
0.842 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
0.998 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Homo sapiens | |
heme | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
3.09 | - |
palmitic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
4.76 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
4.8 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
6.04 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
6.16 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
6.48 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
8.21 | - |
palmitic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
9.27 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
10.72 | - |
myristic acid | pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 | Homo sapiens | |
11.22 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T | Homo sapiens | |
12.27 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens | |
12.29 | - |
lauric acid | pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 | Homo sapiens | |
12.8 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
13.52 | - |
myristic acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
22.76 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 | Homo sapiens | |
23.91 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G | Homo sapiens | |
30.24 | - |
lauric acid | pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 | Homo sapiens |