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Literature summary for 1.14.14.80 extracted from

  • Han, S.; Cha, G.S.; Chun, Y.J.; Lee, C.H.; Kim, D.; Yun, C.H.
    Biochemical analysis of recombinant CYP4A11 allelic variant enzymes W126R, K276T and S353G (2016), Drug Metab. Pharmacokinet., 31, 445-450 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cytochrome b5 increases the enzyme activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP4A11, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
K276T site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
S353G site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
W126R site-directed mutagenesis, the mutant W126R variant cannot be expressed in Escherichia coli. Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, fatty acids binding constants Homo sapiens
0.014
-
palmitic acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens
0.018
-
palmitic acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
0.018
-
myristic acid pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 Homo sapiens
0.019
-
lauric acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.021
-
palmitic acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
0.021
-
lauric acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
0.021
-
lauric acid pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 Homo sapiens
0.022
-
palmitic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.022
-
palmitic acid pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 Homo sapiens
0.023
-
lauric acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
0.026
-
myristic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.029
-
palmitic acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
0.03
-
myristic acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
0.033
-
myristic acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
0.033
-
lauric acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens
0.037
-
myristic acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
0.037
-
lauric acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
0.045
-
myristic acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in heme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q02928
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 58500, recombinant His-tagged wild-type enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
omega-hydroxylase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.055
-
palmitic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.068
-
palmitic acid pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 Homo sapiens
0.088
-
palmitic acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
0.1
-
palmitic acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
0.115
-
palmitic acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens
0.117
-
lauric acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.157
-
myristic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
0.188
-
palmitic acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
0.193
-
myristic acid pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 Homo sapiens
0.258
-
lauric acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
0.258
-
lauric acid pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 Homo sapiens
0.278
-
myristic acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
0.423
-
myristic acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
0.5
-
myristic acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
0.502
-
lauric acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
0.822
-
myristic acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens
0.842
-
lauric acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
0.998
-
lauric acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Homo sapiens
heme
-
Homo sapiens
NADPH
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
palmitic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
3.09
-
palmitic acid pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 Homo sapiens
4.76
-
palmitic acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
4.8
-
palmitic acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
6.04
-
myristic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
6.16
-
lauric acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
6.48
-
palmitic acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
8.21
-
palmitic acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens
9.27
-
myristic acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
10.72
-
myristic acid pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5 Homo sapiens
11.22
-
lauric acid pH 7.4, 37°C, recombinant mutant K276T Homo sapiens
12.27
-
myristic acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens
12.29
-
lauric acid pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5 Homo sapiens
12.8
-
myristic acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
13.52
-
myristic acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
22.76
-
lauric acid pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5 Homo sapiens
23.91
-
lauric acid pH 7.4, 37°C, recombinant mutant S353G Homo sapiens
30.24
-
lauric acid pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5 Homo sapiens