Literature summary for 1.14.14.80 extracted from

Han, S.; Cha, G.S.; Chun, Y.J.; Lee, C.H.; Kim, D.; Yun, C.H.
Biochemical analysis of recombinant CYP4A11 allelic variant enzymes W126R, K276T and S353G (2016), Drug Metab. Pharmacokinet., 31, 445-450 .

Search for more literature for 1.14.14.80

Activating Compound

Activating Compound	Comment	Organism	Structure
cytochrome b5	increases the enzyme activity	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene CYP4A11, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K276T	site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme	Homo sapiens
S353G	site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme	Homo sapiens
W126R	site-directed mutagenesis, the mutant W126R variant cannot be expressed in Escherichia coli.	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics, fatty acids binding constants	Homo sapiens
0.014	-	palmitic acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens
0.018	-	palmitic acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
0.018	-	myristic acid	pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5	Homo sapiens
0.019	-	lauric acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.021	-	palmitic acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
0.021	-	lauric acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
0.021	-	lauric acid	pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5	Homo sapiens
0.022	-	palmitic acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.022	-	palmitic acid	pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5	Homo sapiens
0.023	-	lauric acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
0.026	-	myristic acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.029	-	palmitic acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
0.03	-	myristic acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
0.033	-	myristic acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
0.033	-	lauric acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens
0.037	-	myristic acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
0.037	-	lauric acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
0.045	-	myristic acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	in heme	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lauric acid + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q02928	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Homo sapiens	-
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lauric acid + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	14-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 58500, recombinant His-tagged wild-type enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
omega-hydroxylase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.055	-	palmitic acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.068	-	palmitic acid	pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5	Homo sapiens
0.088	-	palmitic acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
0.1	-	palmitic acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
0.115	-	palmitic acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens
0.117	-	lauric acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.157	-	myristic acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
0.188	-	palmitic acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
0.193	-	myristic acid	pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5	Homo sapiens
0.258	-	lauric acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
0.258	-	lauric acid	pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5	Homo sapiens
0.278	-	myristic acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
0.423	-	myristic acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
0.5	-	myristic acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
0.502	-	lauric acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
0.822	-	myristic acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens
0.842	-	lauric acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
0.998	-	lauric acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Homo sapiens
heme	-	Homo sapiens
NADPH	-	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.5	-	palmitic acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
3.09	-	palmitic acid	pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5	Homo sapiens
4.76	-	palmitic acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
4.8	-	palmitic acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
6.04	-	myristic acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
6.16	-	lauric acid	pH 7.4, 37°C, recombinant wild-type enzyme	Homo sapiens
6.48	-	palmitic acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
8.21	-	palmitic acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens
9.27	-	myristic acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
10.72	-	myristic acid	pH 7.4, 37°C, recombinant wild-type enzyme with cytochrome B5	Homo sapiens
11.22	-	lauric acid	pH 7.4, 37°C, recombinant mutant K276T	Homo sapiens
12.27	-	myristic acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens
12.29	-	lauric acid	pH 7.4, 37°C, recombinant wild-type enzyme, with cytochrome B5	Homo sapiens
12.8	-	myristic acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
13.52	-	myristic acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
22.76	-	lauric acid	pH 7.4, 37°C, recombinant mutant K276T with cytochrome B5	Homo sapiens
23.91	-	lauric acid	pH 7.4, 37°C, recombinant mutant S353G	Homo sapiens
30.24	-	lauric acid	pH 7.4, 37°C, recombinant mutant S353G with cytochrome B5	Homo sapiens

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Release 2023.1 (January 2023)