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Literature summary for 1.14.14.77 extracted from

  • Yamaguchi, T.; Kuwahara, Y.; Asano, Y.
    A novel cytochrome P450, CYP3201B1, is involved in (R)-mandelonitrile biosynthesis in a cyanogenic millipede (2017), FEBS open bio, 7, 335-347 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
coexpressed with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae Chamberlinius hualienensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
2-methyl phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
0.0069
-
3-methyl phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
0.0092
-
phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
0.0158
-
4-Hydroxyphenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 Chamberlinius hualienensis
-
(2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 Chamberlinius hualienensis
-
(2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 Chamberlinius hualienensis
-
(2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information Chamberlinius hualienensis no substrate: (E/Z)-phenylacetaldoxime ?
-
?
additional information Chamberlinius hualienensis no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene ?
-
?
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 Chamberlinius hualienensis
-
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Chamberlinius hualienensis A0A1J1DVP9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
Chamberlinius hualienensis (2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
Chamberlinius hualienensis (2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
Chamberlinius hualienensis (2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information no substrate: (E/Z)-phenylacetaldoxime Chamberlinius hualienensis ?
-
?
additional information no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene Chamberlinius hualienensis ?
-
?
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
Chamberlinius hualienensis (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP3201B1
-
Chamberlinius hualienensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Chamberlinius hualienensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
stable for 20 min Chamberlinius hualienensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.71
-
4-Hydroxyphenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
1.09
-
3-methyl phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
1.12
-
2-methyl phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
3.07
-
phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Chamberlinius hualienensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7
-
Chamberlinius hualienensis

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Chamberlinius hualienensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
45
-
4-Hydroxyphenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
158
-
3-methyl phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
170
-
2-methyl phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis
335
-
phenylacetonitrile pH 6.0, 20°C Chamberlinius hualienensis