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Literature summary for 1.14.14.47 extracted from

  • Holden, J.K.; Lim, N.; Poulos, T.L.
    Identification of redox partners and development of a novel chimeric bacterial nitric oxide synthase for structure activity analyses (2014), J. Biol. Chem., 289, 29437-29445.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.47

Application

Application Comment Organism
analysis development of a robust enzyme assay for measuring NOS activity and inhibition Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type in complex with inhibitors N-omega-nitro-L-arginine and 3-bromo-7-nitroindazole Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information a chimera between enzyme and flavodoxin YkuN is 8fold more active tan wild-type. Adding excess amounts of YkuN does not significantly alter activity Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
3-bromo-7-nitroindazole
-
 Bacillus subtilis
7-nitroindazole
-
 Bacillus subtilis
N-omega-nitro-L-arginine
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34453
-
-
Bacillus subtilis 168 O34453
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 reduced flavodoxin + 4 O2
-
 Bacillus subtilis 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O overall reaction ?
2 L-arginine + 3 reduced flavodoxin + 4 O2
-
 Bacillus subtilis 168 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O overall reaction ?
additional information NO production requires flavodoxin YkuN and a flavodoxin reductase, YumC fulfilling this requirement Bacillus subtilis ?
-
 ?
additional information NO production requires flavodoxin YkuN and a flavodoxin reductase, YumC fulfilling this requirement Bacillus subtilis 168 ?
-
 ?
N-omega-hydroxy-L-arginine + reduced flavodoxin + 2 O2
-
 Bacillus subtilis 2 L-citrulline + 2 nitric oxide + oxidized flavodoxin + 2 H2O
-
 ?
N-omega-hydroxy-L-arginine + reduced flavodoxin + 2 O2
-
 Bacillus subtilis 168 2 L-citrulline + 2 nitric oxide + oxidized flavodoxin + 2 H2O
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0027
-
 chimera between enzyme and flavodoxin YkuN, pH 7.4, 35°C Bacillus subtilis 7-nitroindazole
0.0102
-
 chimera between enzyme and flavodoxin YkuN, pH 7.4, 35°C Bacillus subtilis N-omega-nitro-L-arginine