Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus kaustophilus |
Crystallization (Comment) | Organism |
---|---|
to 3.2 A resolution. Residue Lys-356 (Bacillus subtilis NOS) is changed to Arg-365 (gsNOS), substitution alters the conformation of a conserved Asp carboxylate, resulting in movement of an Ile residue toward the heme | Geobacillus kaustophilus |
Protein Variants | Comment | Organism |
---|---|---|
L356R | mutation Lys-356 (Bacillus subtilis NOS) to Arg-365 (gsNOS) substitution alters the conformation of a conserved Asp carboxylate, resulting in movement of an Ile residue toward the heme | Geobacillus kaustophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
gel filtration | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | Q5KZC5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 2 reduced flavodoxin + 2 O2 | - |
Geobacillus kaustophilus | 2 Nomega-hydroxy-L-arginine + 2 oxidized flavodoxin + 2 H2O | - |
? | |
2 Nomega-hydroxy-L-arginine + reduced flavodoxin + 2 O2 | - |
Geobacillus kaustophilus | 2 L-citrulline + 2 nitric oxide + oxidized flavodoxin + 2 H2O | in single turnover experiments with Nomega-hydroxy-L-arginine, NO forms only in the presence of (6R)-tetrahydro-L-biopterin | ? | |
additional information | addition of oxygen to ferrous NOS results in long-lived heme-oxy complexes in the presence (Soret peak 427 nm) and absence (Soret peak 413 nm) of substrates L-arginine and Nomega-hydroxy-L-arginine. The substrate-induced red shift correlates with hydrogen bonding between substrate and heme-bound oxygen resulting in conversion to a ferric heme-superoxy species | Geobacillus kaustophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, calculated | Geobacillus kaustophilus |
Synonyms | Comment | Organism |
---|---|---|
GK1676 | - |
Geobacillus kaustophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
melting temperature | Geobacillus kaustophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | (6R)-tetrahydro-L-biopterin | Geobacillus kaustophilus |