Literature summary for 1.14.14.47 extracted from

Holden, J.K.; Dejam, D.; Lewis, M.C.; Huang, H.; Kang, S.; Jing, Q.; Xue, F.; Silverman, R.B.; Poulos, T.L.
Inhibitor bound crystal structures of bacterial nitric oxide synthase (2015), Biochemistry, 54, 4075-4082.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with a number of inhibitors	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
E25A/E26A/E316A	mutant facilitates crystallization	Bacillus subtilis
E25A/E26A/E316A/Y357F	mutant facilitates crystallization	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
3-[2,4-di(6-amino-4-methylpyridin-2-yl)ethyl]benzonitrile	inhibitor binds to heme propionate A through a bifurcated H-bond and a pi-pi stacking interaction between the conserved Tyr and aminopyridine group	Bacillus subtilis
6-[5-([4-[(6-amino-4-methylpyridin-2-yl)methyl]pyrrolidin-3-yl]oxy)pentyl]-4-methylpyridin-2-amine	binding is stabilized by a 3.2 A H-bond between the pyrrolidine ring and the carbonyl group of tetrahydrobiopterin	Bacillus subtilis
N,N'-[[(2S)-3-aminopropane-1,2-diyl]bis(oxymethylene-3,1-phenylene)]di(thiophene-2-carboximidamide)	inhibitor binds by extending outside the active site to interact with a surface adjacent to residue Y357	Bacillus subtilis
N1-[6-[2-(6-amino-4-methylpyridin-2-yl)ethyl]pyridin-2-yl]-N1,N2-dimethylethane-1,2-diamine	inhibitor binding distorts the pterin binding site by inducing an alternative rotameric position in residue W329. KS value 18.3 microM	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O34453	-	-
Bacillus subtilis 168	O34453	-	-

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Release 2023.1 (January 2023)