Inhibitors | Comment | Organism | Structure |
---|---|---|---|
n-octylamine | competitive | Manihot esculenta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
(E)-2-methylbutanal oxime | pH 7.9, 28°C | Manihot esculenta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manihot esculenta | Q6XQ14 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endodermis | of nearly unfolded leaf | Manihot esculenta | - |
leaf | in nearly unfolded leaves, the CYP71E7 paralogs are preferentially expressed in specific cells in the endodermis and in most cells in the first cortex cell layer. In fully unfolded leaves, the expression is pronounced in the cortex cell layer just beside the epidermis and in specific cells in the vascular tissue cortex cells | Manihot esculenta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-methylbutanal oxime | - |
Manihot esculenta | (Z)-2-methylbutanal oxime | - |
? | |
(E)-2-methylbutanal oxime + [reduced NADPH-hemoprotein reductase] + O2 | i.e. isoleucine-derived oxime | Manihot esculenta | 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
(E)-2-methylpropanal oxime | - |
Manihot esculenta | (Z)-2-methylpropanal oxime | - |
? | |
(E)-2-methylpropanal oxime + [reduced NADPH-hemoprotein reductase] + O2 | i.e. valine-derived oxime | Manihot esculenta | 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
(Z)-2-methylbutanal oxime | - |
Manihot esculenta | 2-methylbutanenitrile + H2O | - |
? | |
(Z)-2-methylpropanal oxime | - |
Manihot esculenta | 2-methylpropanenitrile + H2O | - |
? | |
2-methylbutanenitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Manihot esculenta | 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
2-methylpropanenitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Manihot esculenta | 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | CYP71E7 exhibits low specificity for the side chain of the substrate and catalyzes the conversion of aliphatic and aromatic oximes | Manihot esculenta | ? | - |
? | |
N-hydroxy-2-(4-hydroxypheny)-lethanimine + [reduced NADPH-hemoprotein reductase] + O2 | i.e. phenylalanine-derived oxime | Manihot esculenta | (4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
N-hydroxy-2-phenylethanimine + [reduced NADPH-hemoprotein reductase] + O2 | i.e. phenylalanine-derived oxime | Manihot esculenta | phenylacetonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? |
Synonyms | Comment | Organism |
---|---|---|
CYP71E7 | - |
Manihot esculenta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N-hydroxy-2-phenylethanimine | pH 7.9, 28°C | Manihot esculenta | |
0.13 | - |
N-hydroxy-2-(4-hydroxypheny)-lethanimine | pH 7.9, 28°C | Manihot esculenta | |
0.28 | - |
(E)-2-methylbutanal oxime | pH 7.9, 28°C | Manihot esculenta | |
0.35 | - |
(E)-2-methylpropanal oxime | pH 7.9, 28°C | Manihot esculenta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Manihot esculenta |
General Information | Comment | Organism |
---|---|---|
physiological function | CYP71E7 is the oxime-metabolizing cytochrome P450 in the biosynthesis of lotaustralin and linamarin. Expression of CYP71E7 in yeast affords microsomes converting 2-methylpropanal oxime and 2-methylbutanal oxime to the corresponding cyanohydrins, which dissociate into acetone and 2-butanone, respectively, and hydrogen cyanide | Manihot esculenta |