Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | reduced glutathione, final concentration of 3 mM, slight stimulation | Arabidopsis thaliana | |
reduced glutathione | addition of reduced glutathione to a final concentration of 3 mM stimulates aldoxime production | Arabidopsis thaliana | |
Tween 80 | addition of Tween 80 to a final concentration of 0.083% (v/v) results in a 1.5fold increase in aldoxime production | Arabidopsis thaliana | |
Tween 80 | final concentration of 0.083% (v/v), 1.5fold increase in aldoxime production | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Arabidopsis thaliana |
expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
L-phenylalanine | pH 7.5, 26°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FLC8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | (E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | overall reaction | ? | |
L-phenylalanine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Arabidopsis thaliana | (E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
additional information | neither L-tyrosine, L-tryptophan, L-methionine, nor DL-homophenylalanine are metabolized by CYP79A2 | Arabidopsis thaliana | ? | - |
? | |
additional information | no substrates: L-tyrosine, L-tryptophan, L-methionine, or DL-homophenylalanine | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
CYP79A2 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
25% of maximum activity | Arabidopsis thaliana |
6 | 7.9 | compared with the activity at pH 7.5, the activity of chimeric CYP79A2 is 25% at pH 6.0, 50% at pH 6.5, 80% at pH 7.0, and 70% at pH 7.9 | Arabidopsis thaliana |
7.9 | - |
70% of maximum activity | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | CYP79A2 is involved in the biosynthesis of benzylglucosinolate | Arabidopsis thaliana |
physiological function | enzyme is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of benzylglucosinolate. Transgenic Arabidopsis thaliana constitutively expressing CYP79A2 accumulate high levels of benzylglucosinolate | Arabidopsis thaliana |