General Stability | Organism |
---|---|
CYP71E1 is labile and prone to rapid denaturation at room temperature. CYP71E1 is isolated in the low spin form. | Sorghum bicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sorghum bicolor | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | O48958 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Sorghum bicolor | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sorghum bicolor | (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction. NADPH is a much better cofactor for NADPH-hemoprotein reductase than NADH although NADH does support the entire catalytic cycle | ? | |
(E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | oxime dervied from phenylalanine, 35% of the activity with (E)-4-hydroxyphenylacetaldehyde oxime | Sorghum bicolor | (S)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
additional information | CYP71E1 metabolizes aromatic oximes efficiently, whereas aliphatic oximes are slowly metabolized | Sorghum bicolor | ? | - |
? |