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Literature summary for 1.14.14.36 extracted from

  • Sibbesen, O.; Koch, B.; Halkier, B.; Moller, B.
    Cytochrome P-450TYR is a multifunctional heme-thiolate enzyme catalyzing the conversion of L-tyrosine to p-hydroxyphenylacetaldehyde oxime in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench (1995), J. Biol. Chem., 270, 3506-3511.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-tyrosine presence of 0.15 mM NaCl, pH 7.9, 25°C Sorghum bicolor
0.21
-
 L-tyrosine pH 7.9, 25°C Sorghum bicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sorghum bicolor 16020
-

Organism

Organism UniProt Comment Textmining
Sorghum bicolor Q43135
-
-

Renatured (Commentary)

Renatured (Comment) Organism
lipid micelles made from L-alpha-dilauroyl phosphatidylcholine are more than twice as effective in reconstituting cytochrome P-450Tyr activity as compared to other lipids Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Sorghum bicolor (E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O overall reaction ?
additional information product of the second N-hydroxylation reaction is N,N-dihydroxytyrosine. N,N-dihydroxytyrosine is dehydrated to 2-nitroso-3-(4-hydroxyphenyl) propionic acid which decarboxylates to p-hydroxyphenylacetaldehyde oxime. The dehydration and decarboxylation reactions may proceed non-enzymatically. The E/Z ratio of the p-hydroxyphenylacetaldehyde oxime produced by reconstituted cytochrome P450Tyr is 69:31. Binding of L-tyrosine or N-hydroxytyrosine mutually excludes binding of the other substrate Sorghum bicolor ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP79A1
-
 Sorghum bicolor
Cytochrome P450Tyr
-
 Sorghum bicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.33
-
 L-tyrosine presence of 0.15 mM NaCl, pH 7.9, 25°C Sorghum bicolor
3.83
-
 L-tyrosine pH 7.9, 25°C Sorghum bicolor