Literature summary for 1.14.14.3 extracted from

Tinikul, R.; Lawan, N.; Akeratchatapan, N.; Pimviriyakul, P.; Chinantuya, W.; Suadee, C.; Sucharitakul, J.; Chenprakhon, P.; Ballou, D.P.; Entsch, B.; Chaiyen, P.
Protonation status and control mechanism of flavin-oxygen intermediates in the reaction of bacterial luciferase (2021), FEBS J., 288, 3246-3260 .

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Protein Variants

Protein Variants	Comment	Organism
H44A	1.5% of wild-type activity	Vibrio harveyi
H44D	2.1% of wild-type activity	Vibrio harveyi
H44N	2.2% of wild-type activity	Vibrio harveyi
H45A	1.7% of wild-type activity	Vibrio harveyi
H4A/H45A	1.95% of wild-type activity	Vibrio harveyi

Organism

Organism	UniProt	Comment	Textmining
Vibrio harveyi	P07740 and P07739	P07740 i.e. subunit LuxA, P07739 i.e. subunit LuxB	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a long-chain aldehyde + FMNH2 + O2	-	Vibrio harveyi	a long-chain fatty acid + FMN + H2O + hv	-	?

General Information

General Information	Comment	Organism
metabolism	during the reaction, the first observed flavin C4a intermediate at pH below 8.5 is the protonated flavin C4a-hydroperoxide, which loses a proton to become an active flavin C4a-peroxide. Residue His44 plays an essential role in deprotonating the flavin C4a-hydroperoxide and initiating chemical catalysis. Residue His45 has a role in binding reduced FMN	Vibrio harveyi

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Release 2023.1 (January 2023)