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Literature summary for 1.14.14.3 extracted from

  • Xu, T.; Ripp, S.; Sayler, G.S.; Close, D.M.
    Expression of a humanized viral 2A-mediated lux operon efficiently generates autonomous bioluminescence in human cells (2014), PLoS ONE, 9, e96347 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine transient and stable transfection of human kidney, breast cancer, and colorectal cancer cell lines by a codon optimized lux expression cassette using viral 2A elements as linker regions. The expression product produces autobioluminescent phenotypes in all cell lines tested without the induction of cytotoxicity and allows for repeated interrogation of populations and self-directed control of bioluminescent activation with detection limits and EC50 values similar to traditional reporter systems Photorhabdus luminescens

Organism

Organism UniProt Comment Textmining
Photorhabdus luminescens P19839 and P19840 P19839 i.e. subunit LuxA, P19840 i.e. subunit LuxB
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General Information

General Information Comment Organism
physiological function transient and stable transfection of human kidney, breast cancer, and colorectal cancer cell lines by a codon optimized lux expression cassette using viral 2A elements as linker regions. The expression product produces autobioluminescent phenotypes in all cell lines tested without the induction of cytotoxicity and allows for repeated interrogation of populations and self-directed control of bioluminescent activation with detection limits and EC50 values similar to traditional reporter systems Photorhabdus luminescens