Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows enhanced affinity for chaperone ClpA | Photorhabdus luminescens | |
additional information | the enzyme shows enhanced affinity for chaperone DnaK | Aliivibrio fischeri |
Cloned (Comment) | Organism |
---|---|
expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively | Aliivibrio fischeri |
expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively | Photorhabdus luminescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RCHO + FMNH2 + O2 | Aliivibrio fischeri | 3-step process via H2O2 as intermediate | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
RCHO + FMNH2 + O2 | Photorhabdus luminescens | 3-step process via H2O2 as intermediate | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | - |
marine bacterium | - |
Photorhabdus luminescens | - |
terrestrial bacterium | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Aliivibrio fischeri |
recombinant enzyme from Escherichia coli | Photorhabdus luminescens |
Renatured (Comment) | Organism |
---|---|
quick refolding within several min at room temperature or 25°C of thermoinactivated enzyme requires ATP and the DnaK-DnaJ-GrpE-system encoding the Hsp70 chaperone but is independent of chaperone ClpA, 80% activity after reactivation, refolding depends on the Escherichia coli strain used for recombinant expression of the luciferase, overview | Aliivibrio fischeri |
slow refolding at room temperature or 35°C of thermoinactivated recominant enzyme requires the DnaK-DnaJ-GrpE-system encoding the Hsp70 chaperone, 7-8% activity after refolding over 10 min, refolding depends on the Escherichia coli strain used for recombinant expression of the luciferase, overview | Photorhabdus luminescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
n-decanal + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Aliivibrio fischeri | n-decanoate + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
n-decanal + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Photorhabdus luminescens | n-decanoate + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
RCHO + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Aliivibrio fischeri | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
RCHO + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Photorhabdus luminescens | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir |
Synonyms | Comment | Organism |
---|---|---|
bacterial luciferase | - |
Aliivibrio fischeri |
bacterial luciferase | - |
Photorhabdus luminescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Aliivibrio fischeri |
35 | - |
- |
Photorhabdus luminescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme shows reduced thermal stability in absence of chaperone Hsp100 after expression in an Escherichia coli clpA-mutant strain, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo | Photorhabdus luminescens |
additional information | - |
the recombinant enzyme shows unaffected thermal stability after expression in an Escherichia coli clpA-mutant strain lacking chaperone Hsp100, carbonyl cyanide 3-chlorophenylhydrazone highly decreases the thermal stability of the enzyme in vivo to in vitro level, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo | Aliivibrio fischeri |
43.5 | - |
inactivation in vitro, temperature-stable enzyme | Photorhabdus luminescens |
43.5 | - |
inactivation, temperature-labile enzyme | Aliivibrio fischeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aliivibrio fischeri |
7 | - |
assay at | Photorhabdus luminescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Aliivibrio fischeri | |
FMNH2 | - |
Photorhabdus luminescens |