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Literature summary for 1.14.14.3 extracted from

  • Lei, B.; Ding, Q.; Tu, S.C.
    Identity of the emitter in the bacterial luciferase luminescence reaction: binding and fluorescence quantum yield studies of 5-decyl-4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate as a model (2004), Biochemistry, 43, 15975-15982.
    View publication on PubMed
Search for more literature for 1.14.14.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant C106A enzymes in Escherichia coli strain JM101 Vibrio harveyi

Protein Variants

Protein Variants Comment Organism
C106A site-directed mutagenesis, catalytic properties are similar to the wild-type enzyme, mutant shows 60% of wild-type quantum yield Vibrio harveyi

Inhibitors

Inhibitors Comment Organism Structure
5-decyl-4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate binding and fluorescence quantum yield studies of the substance as a model, complexed with the enzyme in a 1:1 molcular ratio leading to 80% and 90% inhibition of wild-type and mutant C106A at 0.01 mM, respectively, binds to the active site Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant C106A enzymes from Escherichia coli strain JM101 to over 95% purity Vibrio harveyi

Reaction

Reaction Comment Organism Reaction ID
a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu reaction mechanism and scheme, cofactor binding Vibrio harveyi
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanal + FMNH2 + O2
-
 Vibrio harveyi decanoate + FMN + H2O + hn
-
 ir

Synonyms

Synonyms Comment Organism
bacterial luciferase
-
 Vibrio harveyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Vibrio harveyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate model bioluminescence emitter molecule, binding and fluorescence quantum yield studies, complexed with the enzyme in a 1:1 molcular ratio Vibrio harveyi
FMNH2 physiological cofactor Vibrio harveyi