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Literature summary for 1.14.14.25 extracted from

  • Mast, N.; Liao, W.L.; Pikuleva, I.A.; Turko, I.V.
    Combined use of mass spectrometry and heterologous expression for identification of membrane-interacting peptides in cytochrome P450 46A1 and NADPH-cytochrome P450 oxidoreductase (2009), Arch. Biochem. Biophys., 483, 81-89.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
full-length and truncated (DELTA2-50) CYP46A1 expressed in Escherichia coli membranes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y6A2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 cholesterol enters the enzyme through the membrane. In CYP46A1, the three putative membrane-interacting peptides in the soluble domain, 59V-K69, 77V-K94, and 217L-K231, form the entrance to the substrate access channel and represent the secondary structural elements (the A helix and beta1 sheet and F-G loop regions) Homo sapiens (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP46A1
-
 Homo sapiens
cytochrome P450 46A1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function recombinant CYP46A1 is catalytically active when associated with Escherichia coli membranes Homo sapiens