Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.24 extracted from

  • Strushkevich, N.; Usanov, S.A.; Plotnikov, A.N.; Jones, G.; Park, H.W.
    Structural analysis of CYP2R1 in complex with vitamin D3 (2008), J. Mol. Biol., 380, 95-106.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expresion in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with vitamin D3, to 1.0 A resolution. The CYP2R1 structure adopts a closed conformation with the substrate access channel being covered by the ordered B'-helix and slightly opened to the surface, which defines the substrate entrance point. The active site is lined by conserved, mostly hydrophobic residues. Vitamin D3 is bound in an elongated conformation with the aliphatic side-chain pointing toward the heme. The structure reveals the secosteroid binding mode in an extended active site Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
vitamin D3 pH 7.4, 37°C Homo sapiens
0.0113
-
1alpha-hydroxyvitamin D3 pH 7.4, 37°C Homo sapiens
0.0158
-
1alpha-hydroxyvitamin D2 pH 7.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6VVX0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1alpha-hydroxyvitamin D2 + O2 + NADPH + H+
-
Homo sapiens 1alpha,25-dihydroxyvitamin D2 + NADP+ + H2O
-
?
1alpha-hydroxyvitamin D3 + O2 + NADPH + H+
-
Homo sapiens 1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
?
additional information no substrates: 25-hydroxyvitamin D3, cholesterol, 7-dehydrocholesterol Homo sapiens ?
-
?
vitamin D3 + O2 + NADPH + H+
-
Homo sapiens 25-hydroxyvitamin D3 + NADP+ + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0075
-
1alpha-hydroxyvitamin D3 pH 7.4, 37°C Homo sapiens
0.008
-
vitamin D3 pH 7.4, 37°C Homo sapiens
0.02
-
1alpha-hydroxyvitamin D2 pH 7.4, 37°C Homo sapiens