Cloned (Comment) | Organism |
---|---|
expresion in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with vitamin D3, to 1.0 A resolution. The CYP2R1 structure adopts a closed conformation with the substrate access channel being covered by the ordered B'-helix and slightly opened to the surface, which defines the substrate entrance point. The active site is lined by conserved, mostly hydrophobic residues. Vitamin D3 is bound in an elongated conformation with the aliphatic side-chain pointing toward the heme. The structure reveals the secosteroid binding mode in an extended active site | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
vitamin D3 | pH 7.4, 37°C | Homo sapiens | |
0.0113 | - |
1alpha-hydroxyvitamin D3 | pH 7.4, 37°C | Homo sapiens | |
0.0158 | - |
1alpha-hydroxyvitamin D2 | pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6VVX0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha-hydroxyvitamin D2 + O2 + NADPH + H+ | - |
Homo sapiens | 1alpha,25-dihydroxyvitamin D2 + NADP+ + H2O | - |
? | |
1alpha-hydroxyvitamin D3 + O2 + NADPH + H+ | - |
Homo sapiens | 1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
additional information | no substrates: 25-hydroxyvitamin D3, cholesterol, 7-dehydrocholesterol | Homo sapiens | ? | - |
? | |
vitamin D3 + O2 + NADPH + H+ | - |
Homo sapiens | 25-hydroxyvitamin D3 + NADP+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
1alpha-hydroxyvitamin D3 | pH 7.4, 37°C | Homo sapiens | |
0.008 | - |
vitamin D3 | pH 7.4, 37°C | Homo sapiens | |
0.02 | - |
1alpha-hydroxyvitamin D2 | pH 7.4, 37°C | Homo sapiens |