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Literature summary for 1.14.14.24 extracted from

  • Masumoto, O.; Ohyama, Y.; Okuda, K.
    Purification and characterization of vitamin D 25-hydroxylase from rat liver mitochondria (1988), J. Biol. Chem., 263, 14256-14260.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Metyrapone slight inhibition Rattus norvegicus
additional information not inhibitory: aminoglutethimide. The enzyme activity was markedly inhibited in an atmosphere of CO:Os:Nz, 40:20:40 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
1alpha-hydroxycholecalciferol pH 7.4, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52500
-
x * 52500, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1alpha-hydroxycholecalciferol + O2 + NADPH + H+
-
Rattus norvegicus 1alpha,25-dihydroxycholecalciferol + NADP+ + H2O
-
?
cholecalciferol + O2 + NADPH + H+ at about 20% of the rate with 1alpha-hydroxycholecalciferol Rattus norvegicus 25-hydroxycholecalciferol + NADP+ + H2O
-
?
additional information no substrates: benzphetamine, 7-ethoxycoumarin, and benzo[a]pyrene Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 52500, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.063
-
1alpha-hydroxycholecalciferol pH 7.4, 37°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Rattus norvegicus
additional information activity is completely lost when the electron-transferring system is replaced by that of microsomes, i.e. NADPH-cytochrome P-450 reductase purified from rat liver microsomes. The omission of cytochrome P-450, adrenodoxin, or NADPH-adrenodoxin reductase results in complete loss of enzyme activity Rattus norvegicus
NADPH
-
Rattus norvegicus