Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
A241G/L243V/F244L/P245R/R246F | mutation in substrate recognition site 3, to the equivalent residues in CYP2D6, an enzyme not active in 25-hydroxylation. The 25-hydroxylase activity of the mutant is completely lost whereas the activity toward tolterodine remains virtually unaffected | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
bufuralol | pH 7.4, 37°C, mutant A241G/L243V/F244L/P245R/R246F | Sus scrofa | |
0.0014 | - |
bufuralol | pH 7.4, 37°C, wild-type | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha-hydroxyvitamin D3 + O2 + NADPH + H+ | - |
Sus scrofa | 1alpha,25-dihydroxy-vitamin D3 + NADP+ + H2O | - |
? | |
bufuralol + O2 + NADPH + H+ | - |
Sus scrofa | 1'-hydroxybufuralol + NADP+ + H2O | - |
? | |
tolterodine + O2 + NADPH + H+ | - |
Sus scrofa | ? + NADP+ + H2O | - |
? | |
vitamin D3 + O2 + NADPH + H+ | - |
Sus scrofa | 25-hydroxyvitamin D3 + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP2D25 | - |
Sus scrofa |