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Literature summary for 1.14.14.23 extracted from
- Cholesterol 7alpha-hydroxylase is phosphorylated at multiple amino acids (2005), Biochem. Biophys. Res. Commun., 329, 957-965.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), stable expression of CYP7A cDNA using the cytomegalovirus promoter and the bovine growth hormone polyadenylation signal in Hep-G2 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| taurochenodeoxycholate | 70% inhibition at 0.1 mM | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, approximately, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P22680 | gene CYP7A1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme is phosphorylated at multiple amino acids, overview | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant enzyme partially by microsome preparation | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000034 | - |
wild-type Hep-G2 cell microsomes | Homo sapiens |
| 0.000092 | - |
recombinant enzyme in transformed Hep-G2 cells | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, approximately, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cholesterol 7alpha-hydroxylase | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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