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Literature summary for 1.14.14.23 extracted from

  • Tanaka, S.; Kinowaki, M.; Eto, T.A.; Ota, Y.; Maeda, Y.; Okuda, K.; Setoguchi, T.; Chijiiwa, K.
    Sulfhydryl groups responsible for extreme lability of human cholesterol 7alpha-hydroxylase identified by site-directed mutagenesis (2003), J. Steroid Biochem. Mol. Biol., 86, 35-40.
    View publication on PubMed
Search for more literature for 1.14.14.23

Cloned(Commentary)

Cloned (Comment) Organism
native and mutated genes expressed in COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C16A strongly decreased activity Homo sapiens
C175A decreased activity Homo sapiens
C17A increased activity Homo sapiens
C444A no activity Homo sapiens
C44A decreased activity Homo sapiens
C476A no activity Homo sapiens
C69A no effect on activity Homo sapiens
C90A decreased activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Cricetulus griseus first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Mus musculus first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Oryctolagus cuniculus first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Cricetulus griseus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Mus musculus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryctolagus cuniculus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Cricetulus griseus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Mus musculus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Oryctolagus cuniculus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
cholesterol-NADPH oxidoreductase, 7alpha-hydroxylating
-
 Cricetulus griseus
cholesterol-NADPH oxidoreductase, 7alpha-hydroxylating
-
 Mus musculus
cholesterol-NADPH oxidoreductase, 7alpha-hydroxylating
-
 Homo sapiens
cholesterol-NADPH oxidoreductase, 7alpha-hydroxylating
-
 Rattus norvegicus
cholesterol-NADPH oxidoreductase, 7alpha-hydroxylating
-
 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Cricetulus griseus
NADPH
-
 Mus musculus
NADPH
-
 Homo sapiens
NADPH
-
 Rattus norvegicus
NADPH
-
 Oryctolagus cuniculus