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Literature summary for 1.14.14.23 extracted from

  • Kinowaki, M.; Tanaka, S.; Maeda, Y.; Higashi, S.; Okuda, K.; Setoguchi, T.
    Half-life of cholesterol 7alpha-hydroxylase activity and enzyme mass differ in animals and humans when determined by a monoclonal antibody against human cholesterol 7alpha-hydroxylase (2002), J. Steroid Biochem. Mol. Biol., 81, 377-380.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Homo sapiens

General Stability

General Stability Organism
rapid inactivation upon incubating microsomes with NADPH, half-life: 1 h, addition of dithiothreitol partially protects against inactivation Homo sapiens
rapid inactivation upon incubating microsomes with NADPH, half-life: 1 h, addition of dithiothreitol partially protects against inactivation Rattus norvegicus
rapid inactivation upon incubating microsomes with NADPH, half-life: 1 h, addition of dithiothreitol partially protects against inactivation Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-
microsome
-
Oryctolagus cuniculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Homo sapiens
58000
-
x * 58000, SDS-PAGE Rattus norvegicus
58000
-
x * 58000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Oryctolagus cuniculus first step of cholesterol catabolism 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
Oryctolagus cuniculus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 first step of cholesterol catabolism Oryctolagus cuniculus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Homo sapiens
? x * 58000, SDS-PAGE Rattus norvegicus
? x * 58000, SDS-PAGE Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus
NADPH
-
Oryctolagus cuniculus