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Literature summary for 1.14.14.22 extracted from

  • Konishi, J.; Maruhashi, K.
    Residue 345 of dibenzothiophene (DBT) sulfone monooxygenase is involved in C-S bond cleavage specificity of alkylated DBT sulfones (2003), Biotechnol. Lett., 25, 1199-1202.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene dszA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109 Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
L346A site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme Rhodococcus erythropolis
Q345A site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone Rhodococcus erythropolis
T344A site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme Rhodococcus erythropolis
T344A/Q345A site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Rhodococcus erythropolis
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Rhodococcus erythropolis IGTS8 / ATCC 53968
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
gene dszA
-
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
gene dszA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 activity of mutant Q345A Rhodococcus erythropolis ? + 2 FMN + H2O
-
?
3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 activity of mutant Q345A Rhodococcus erythropolis IGTS8 / ATCC 53968 ? + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Rhodococcus erythropolis 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Rhodococcus erythropolis IGTS8 / ATCC 53968 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
additional information no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes Rhodococcus erythropolis ?
-
?
additional information no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes Rhodococcus erythropolis IGTS8 / ATCC 53968 ?
-
?

Synonyms

Synonyms Comment Organism
DBT sulfone monooxygenase
-
Rhodococcus erythropolis
dibenzothiophene sulfone monooxygenase
-
Rhodococcus erythropolis
dszA
-
Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Rhodococcus erythropolis

General Information

General Information Comment Organism
additional information residue Q345 is involved in C-S bond cleavage specifically for alkylated dibenzothiophene sulfone Rhodococcus erythropolis