Cloned (Comment) | Organism |
---|---|
gene dszA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
L346A | site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme | Rhodococcus erythropolis |
Q345A | site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone | Rhodococcus erythropolis |
T344A | site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme | Rhodococcus erythropolis |
T344A/Q345A | site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
gene dszA | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
gene dszA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | activity of mutant Q345A | Rhodococcus erythropolis | ? + 2 FMN + H2O | - |
? | |
3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | activity of mutant Q345A | Rhodococcus erythropolis IGTS8 / ATCC 53968 | ? + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
additional information | no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes | Rhodococcus erythropolis IGTS8 / ATCC 53968 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
dibenzothiophene sulfone monooxygenase | - |
Rhodococcus erythropolis |
dszA | - |
Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
additional information | residue Q345 is involved in C-S bond cleavage specifically for alkylated dibenzothiophene sulfone | Rhodococcus erythropolis |