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Literature summary for 1.14.14.21 extracted from

  • Ohshiro, T.; Nakura, S.; Ishii, Y.; Kino, K.; Kirimura, K.; Izumi, Y.
    Novel reactivity of dibenzothiophene monooxygenase from Bacillus subtilis WU-S2B (2009), Biosci. Biotechnol. Biochem., 73, 2128-2130.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bdsC, recombinant expression in Escherichia coli strain BL21(DE3)/pET21-a/bdsC Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis WU-S2B
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene bdsC
-
Bacillus subtilis WU-S2B
-
gene bdsC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,7-diethylbenzothiophene + FMNH2 + O2 13% of the activity with dibenzothiophene Bacillus subtilis ?
-
?
2,7-diethylbenzothiophene + FMNH2 + O2 13% of the activity with dibenzothiophene Bacillus subtilis WU-S2B ?
-
?
2-ethylbenzothiophene + FMNH2 + O2 28% of the activity with dibenzothiophene Bacillus subtilis ?
-
?
2-ethylbenzothiophene + FMNH2 + O2 28% of the activity with dibenzothiophene Bacillus subtilis WU-S2B ?
-
?
2-methylbenzothiophene + FMNH2 + O2 209% of the activity with dibenzothiophene Bacillus subtilis ?
-
?
2-methylbenzothiophene + FMNH2 + O2 209% of the activity with dibenzothiophene Bacillus subtilis WU-S2B ?
-
?
3-methylbenzothiophene + FMNH2 + O2 less than 10% of the activity with dibenzothiophene Bacillus subtilis ?
-
?
5-methylbenzothiophene + FMNH2 + O2 33% of the activity with dibenzothiophene Bacillus subtilis ?
-
?
7-ethylbenzothiophene + FMNH2 + O2 less than 10% of the activity with dibenzothiophene Bacillus subtilis ?
-
?
dibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis WU-S2B dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
additional information the enzyme utilizes aromatic compounds, not having sulfur atoms, as substrates. It acts on indole, converting it to indoxyl and isatin, and its derivatives to form indigoid pigments, and also utilizes indoline and phenoxazine. Indigo derivative formation by BdsC using methylindoles as substrates. Enzyme BdsC exhibits activity toward benzothiophene derivatives but not benzothiophene itself showing a wide reactivity toward aromatic compounds. To measure BdsC activity, flavin reductase (DszD) from another desulfurizing species, Rhodococcus erythropolis strain D-1, is used. Sulfone formations, product analysis by GC-MS Bacillus subtilis ?
-
?
additional information the enzyme utilizes aromatic compounds, not having sulfur atoms, as substrates. It acts on indole, converting it to indoxyl and isatin, and its derivatives to form indigoid pigments, and also utilizes indoline and phenoxazine. Indigo derivative formation by BdsC using methylindoles as substrates. Enzyme BdsC exhibits activity toward benzothiophene derivatives but not benzothiophene itself showing a wide reactivity toward aromatic compounds. To measure BdsC activity, flavin reductase (DszD) from another desulfurizing species, Rhodococcus erythropolis strain D-1, is used. Sulfone formations, product analysis by GC-MS Bacillus subtilis WU-S2B ?
-
?

Synonyms

Synonyms Comment Organism
BdsC
-
Bacillus subtilis
dibenzothiophene monooxygenase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bacillus subtilis

General Information

General Information Comment Organism
metabolism the dibenzothiophene (DBT) desulfurization enzyme system consists of three enzymes: DBT monooxygenase, DBT sulfone monooxygenase, and 2'-hydroxybiphenyl 2-sulfinate desulfinase Bacillus subtilis