Cloned (Comment) | Organism |
---|---|
gene tdsC, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli, coexpression with genes tdsC and tdsB, leading to established thermophilic desulfurization of dibenzothiophene in Escherichia coli host, an Escherichia coli oxidoreductase can be functionally coupled with the monooxygenases of a gram-positive thermophile | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45800 | - |
x * 45800, about, sequnence calculation | Paenibacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | Paenibacillus sp. | overall reaction | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | Paenibacillus sp. A11-2 | overall reaction | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + FMNH2 + O2 | Paenibacillus sp. | - |
dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
dibenzothiophene + FMNH2 + O2 | Paenibacillus sp. A11-2 | - |
dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
dibenzothiophene-5-oxide + FMNH2 + O2 | Paenibacillus sp. | - |
dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? | |
dibenzothiophene-5-oxide + FMNH2 + O2 | Paenibacillus sp. A11-2 | - |
dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | Q9LBX2 | gene tdsC | - |
Paenibacillus sp. A11-2 | Q9LBX2 | gene tdsC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | overall reaction | Paenibacillus sp. | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | overall reaction | Paenibacillus sp. A11-2 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + FMNH2 + O2 | - |
Paenibacillus sp. | dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
dibenzothiophene + FMNH2 + O2 | - |
Paenibacillus sp. A11-2 | dibenzothiophene-5-oxide + FMN + H2O | - |
? | |
dibenzothiophene-5-oxide + FMNH2 + O2 | - |
Paenibacillus sp. | dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? | |
dibenzothiophene-5-oxide + FMNH2 + O2 | - |
Paenibacillus sp. A11-2 | dibenzothiophene-5,5-dioxide + FMN + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45800, about, sequnence calculation | Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | assay at, cell extract | Paenibacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Paenibacillus sp. |