Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme A105L in complex with progesterone, pregnenolone, 17alpha-hydroxypregnenolone, or abiraterone is crystallized by the hanging drop vapor diffusion method using 100 mM Tris-HCl, pH 8.5, 25% (w/v) PEG 4000, 150 mM magnesium chloride hexahydrate, and 4-6% (v/v) glycerol. Mutant enzyme A105L in complex with 17alpha-hydroxyprogesterone is crystallized by the hanging drop vapor diffusion method using 175 mM Tris-HCl, pH 8.5, 30% (w/v) PEG 3350, 250 mM lithium sulfate, and 3% (v/v) glycerol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A105L | the effect of the A105L mutation is to increase progesterone affinity at least 2fold but the mutant has little effect on the already lower affinity for either progesterone hydroxylation product | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
abiraterone | - |
Homo sapiens | |
TOK-001 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00038 | - |
pregnenolone | mutant enzyme A105L, at pH 7.4 and 37°C | Homo sapiens | |
0.00093 | - |
pregnenolone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.00196 | - |
progesterone | mutant enzyme A105L, at pH 7.4 and 37°C | Homo sapiens | |
0.0105 | - |
progesterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a C21-steroid + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
a 17alpha-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | Homo sapiens | a minor conformation may yield the minor 16alpha-hydroxyprogesterone metabolite from progesterone | ? | - |
? | |
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05093 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid-agarose resin column chromatography, carboxymethyl-Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a C21-steroid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | a 17alpha-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | a minor conformation may yield the minor 16alpha-hydroxyprogesterone metabolite from progesterone | Homo sapiens | ? | - |
? | |
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP17A1 | - |
Homo sapiens |
steroidogenic cytochrome P450 17A1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
pregnenolone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.0065 | - |
pregnenolone | mutant enzyme A105L, at pH 7.4 and 37°C | Homo sapiens | |
0.017 | - |
progesterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.019 | - |
progesterone | mutant enzyme A105L, at pH 7.4 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens |