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Literature summary for 1.14.14.19 extracted from

  • Nakajin, S.; Shinoda, M.; Haniu, M.; Shively, J.E.; Hall, P.F.
    C21 steroid side chain cleavage enzyme from porcine adrenal microsomes. Purification and characterization of the 17alpha-hydroxylase/C17,20-lyase cytochrome P-450 (1984), J. Biol. Chem., 259, 3971-3976.
    View publication on PubMed
Search for more literature for 1.14.14.19

Inhibitors

Inhibitors Comment Organism Structure
sodium cholate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 pregnenolone adrenal gland Sus scrofa
0.0009
-
 17alpha-hydroxypregnenolone adrenal gland Sus scrofa
0.0018
-
 progesterone adrenal gland Sus scrofa
0.0025
-
 17alpha-hydroxyprogesterone adrenal gland Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ heme content more than 0.8 nM/nM protein Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pregnenolone + AH2 + O2 Sus scrofa
-
 17alpha-hydroxypregnenolone + A + H2O product eliminates at C20,21 acetate to yield dehydroepiandrosterone ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2 Sus scrofa
-
 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O product eliminates at C20,21 acetate to yield androstenedione ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland little lyase activity Sus scrofa
-
testis greater lyase than hydroxylase activity Sus scrofa
-

Storage Stability

Storage Stability Organism
-70°C, 0.2% Emulgen 913 v/v and 20% glycerol v/v, 6 months, no detectable loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pregnenolone + AH2 + O2
-
 Sus scrofa 17alpha-hydroxypregnenolone + A + H2O product eliminates at C20,21 acetate to yield dehydroepiandrosterone ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Sus scrofa 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O product eliminates at C20,21 acetate to yield androstenedione ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Sus scrofa