Cloned (Comment) | Organism |
---|---|
gene chuZ, ChuZ is highly conserved in 32 clinical isolates. Genotyping, recombinant expression in Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme complexed with hemin, hemin:ChuZ ratio of 2:1, microbatch method, mixing of 0..001 ml of 170 mg/ml ChuZ-hemin in 20 mM Tris-HCl, pH 8.0, and 5 mM sodium azide with 0.001 ml of reservoir solution consisting of 0.1 M MES, pH 6.5, 24% PEG400 v/v, and 0.1 M imidazole, X-ray diffraction structure determination and analysis at 2.5 A resolution | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + 3 AH2 + 3 O2 | Campylobacter jejuni | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene chuZ | - |
Purification (Comment) | Organism |
---|---|
recombinant ChuZ from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + 3 AH2 + 3 O2 | - |
Campylobacter jejuni | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
heme + 3 reduced ascorbate + 3 O2 | - |
Campylobacter jejuni | biliverdin + Fe2+ + CO + 3 oxidized ascorbate + 3 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | with a splitbarrel fold. One heme-binding site is at the dimer interface and another novel heme-binding site is found on the protein surface | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
ChuZ | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation of ChuZ abolishes the ability of Campylobacter jejuni to use hemin or hemoglobin as sources of iron | Campylobacter jejuni |
additional information | heme is bound in its binding site on the dimer interface by four histidine side-chains through hydrophobic interactions, canonical heme-binding site structure, reaction mechanism and structure-function relationship, overview | Campylobacter jejuni |
physiological function | the enzyme enables the organism to use heme as the sole iron source | Campylobacter jejuni |