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Literature summary for 1.14.14.18 extracted from

  • Zhang, R.; Zhang, J.; Guo, G.; Mao, X.; Tong, W.; Zhang, Y.; Wang, D.C.; Hu, Y.; Zou, Q.
    Crystal structure of Campylobacter jejuni ChuZ: a split-barrel family heme oxygenase with a novel heme-binding mode (2011), Biochem. Biophys. Res. Commun., 415, 82-87.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene chuZ, ChuZ is highly conserved in 32 clinical isolates. Genotyping, recombinant expression in Escherichia coli strain BL21(DE3) Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme complexed with hemin, hemin:ChuZ ratio of 2:1, microbatch method, mixing of 0..001 ml of 170 mg/ml ChuZ-hemin in 20 mM Tris-HCl, pH 8.0, and 5 mM sodium azide with 0.001 ml of reservoir solution consisting of 0.1 M MES, pH 6.5, 24% PEG400 v/v, and 0.1 M imidazole, X-ray diffraction structure determination and analysis at 2.5 A resolution Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heme + 3 AH2 + 3 O2 Campylobacter jejuni
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
gene chuZ
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ChuZ from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heme + 3 AH2 + 3 O2
-
Campylobacter jejuni biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 reduced ascorbate + 3 O2
-
Campylobacter jejuni biliverdin + Fe2+ + CO + 3 oxidized ascorbate + 3 H2O
-
?

Subunits

Subunits Comment Organism
homodimer with a splitbarrel fold. One heme-binding site is at the dimer interface and another novel heme-binding site is found on the protein surface Campylobacter jejuni

Synonyms

Synonyms Comment Organism
ChuZ
-
Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Campylobacter jejuni

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
Campylobacter jejuni

General Information

General Information Comment Organism
malfunction mutation of ChuZ abolishes the ability of Campylobacter jejuni to use hemin or hemoglobin as sources of iron Campylobacter jejuni
additional information heme is bound in its binding site on the dimer interface by four histidine side-chains through hydrophobic interactions, canonical heme-binding site structure, reaction mechanism and structure-function relationship, overview Campylobacter jejuni
physiological function the enzyme enables the organism to use heme as the sole iron source Campylobacter jejuni