Literature summary for 1.14.14.178 extracted from

Kappler, C.; Kabbouh, M.; Hetru, C.; Durst, F.; Hoffmann, J.A.
Characterization of three hydroxylases involved in the final steps of biosynthesis of the steroid hormone ecdysone in Locusta migratoria (Insecta, Orthoptera) (1988), J. Steroid Biochem., 31, 891-898.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2,22,25-trideoxyecdysone	competitive	Locusta migratoria
2-deoxyecdysone	competitive	Locusta migratoria
ecdysone	competitive	Locusta migratoria

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	2,22-dideoxyecdysone	pH 7.2, 37°C	Locusta migratoria

Organism

Organism	UniProt	Comment	Textmining
Locusta migratoria	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,22-dideoxyecdysone + NADPH + H+ + O2	-	Locusta migratoria	? + NADP+ + H2O	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Locusta migratoria

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	-	Locusta migratoria

Cofactor

Cofactor	Comment	Organism	Structure
NADH	20% of the efficiency of NADPH plus a regenerating system	Locusta migratoria
NADPH	-	Locusta migratoria

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.003	-	2,22,25-trideoxyecdysone	pH 7.2, 37°C	Locusta migratoria
0.006	-	2-deoxyecdysone	pH 7.2, 37°C	Locusta migratoria
0.007	-	ecdysone	pH 7.2, 37°C	Locusta migratoria

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Release 2023.1 (January 2023)