Literature summary for 1.14.14.176 extracted from

Rouck, J.E.; Biggs, B.W.; Kambalyal, A.; Arnold, W.R.; De Mey, M.; Ajikumar, P.K.; Das, A.
Heterologous expression and characterization of plant taxadiene-5alpha-hydroxylase (CYP725A4) in Escherichia coli (2017), Protein Expr. Purif., 132, 60-67 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Taxus cuspidata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Taxus cuspidata	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2	Taxus cuspidata	-	taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Taxus cuspidata	Q6WG30	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Taxus cuspidata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2	-	Taxus cuspidata	taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP725A4	-	Taxus cuspidata
taxadiene-5alpha-hydroxylase	-	Taxus cuspidata

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Release 2023.1 (January 2023)