Literature summary for 1.14.14.176 extracted from

Jennewein, S.; Long, R.M.; Williams, R.M.; Croteau, R.
Cytochrome p450 taxadiene 5alpha-hydroxylase, a mechanistically unusual monooxygenase catalyzing the first oxygenation step of taxol biosynthesis (2004), Chem. Biol., 11, 379-387.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.016	-	taxa-4(20),11(12)-diene	pH 7.5	Taxus cuspidata
0.024	-	taxa-4(5),11(12)-diene	pH 7.5	Taxus cuspidata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Taxus cuspidata	-	-

Organism

Organism	UniProt	Comment	Textmining
Taxus cuspidata	Q6WG30	expression in yeast and Sf9 system	-

Reaction

Reaction	Comment	Organism	Reaction ID
taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 = taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	mechanism involving promiscuous radical abstraction with selective oxygen insertion	Taxus cuspidata	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
taxa-4(20),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2	preferred substrate	Taxus cuspidata	taxa-4(20),11(12)-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
taxa-4(5),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2	-	Taxus cuspidata	taxa-4(20),11(12)-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

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Release 2023.1 (January 2023)