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Literature summary for 1.14.14.170 extracted from

  • Gou, J.; Hao, F.; Huang, C.; Kwon, M.; Chen, F.; Li, C.; Liu, C.; Ro, D.K.; Tang, H.; Zhang, Y.
    Discovery of a non-stereoselective cytochrome P450 catalyzing either 8alpha- or 8beta-hydroxylation of germacrene A acid from the Chinese medicinal plant, Inula hupehensis (2018), Plant J., 93, 92-106 .
    View publication on PubMed
Search for more literature for 1.14.14.170

Cloned(Commentary)

Cloned (Comment) Organism
expression in yeast Inula hupehensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Inula hupehensis the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Inula hupehensis
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Purification (Commentary)

Purification (Comment) Organism
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 Inula hupehensis

Source Tissue

Source Tissue Comment Organism Textmining
flower the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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leaf the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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root the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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stem the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone Inula hupehensis 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide Inula hupehensis 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 overall reaction. The product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide Inula hupehensis 8-epi-inunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O
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 ?

Synonyms

Synonyms Comment Organism
CYP71BL6
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 Inula hupehensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
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 assay at Inula hupehensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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 assay at Inula hupehensis

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 heme-thiolate protein Inula hupehensis

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone Inula hupehensis