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Literature summary for 1.14.14.17 extracted from

  • Ono, T.; Nakazono, K.; Kosaka, H.
    Purification and partial characterization of squalene epoxidase from rat liver microsomes (1982), Biochim. Biophys. Acta, 709, 84-90.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is not a cytochrome P-450 enzyme Rattus norvegicus
additional information reconstitution of squalene epoxidase activity by addition of: NADPH-cytochrome P-450 reductase, EC 1.6.2.4, FAD and Triton X-100 Rattus norvegicus

General Stability

General Stability Organism
freezing and thawing once, 20% loss of activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
FAD
-
Rattus norvegicus
0.013
-
squalene
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
sucrose density gradient centrifugation Rattus norvegicus
51000
-
1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of purified enzyme with the addition of NADPH-cytochrome P450 reductase, FAD and Triton X-100 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00619
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, 20 mM Tris-HCl buffer, pH 7.4, 0.5% Triton X-100, several weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene + FAD + H2O
-
Rattus norvegicus squalene + FADH2 + O2
-
r
squalene + NAD(P)H + O2
-
Rattus norvegicus (S)-squalene-2,3-epoxide + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 51000, most of the enzyme behaves as a monomer, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0055
-
squalene
-
Rattus norvegicus