Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.167 extracted from

  • Dang, T.; Chen, X.; Facchini, P.
    Acetylation serves as a protective group in noscapine biosynthesis in opium poppy (2015), Nat. Chem. Biol., 11, 104-106 .
    View publication on PubMed
Search for more literature for 1.14.14.167

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0049
-
 (13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine pH 7.5, 30°C Papaver somniferum
0.0106
-
 (S)-1-hydroxy-N-methylcanadine pH 7.5, 30°C Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine (13S,14R)-13-O-acetyl-1,8-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Papaver somniferum I3V6B7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum (13S,14R)-13-O-acetyl-1,8-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme does not accept (13S,14R)-1,13-dihydroxy-N-methylcanadine Papaver somniferum (13S,14R)-13-O-acetyl-1,8-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum ?
-
 ?

Synonyms

Synonyms Comment Organism
1-hydroxy-13-O-acetyl-N-methylcanadine 8-hydroxylase
-
 Papaver somniferum
CYP82X1
-
 Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 heme-thiolate enzyme Papaver somniferum

General Information

General Information Comment Organism
metabolism the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum