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Literature summary for 1.14.14.162 extracted from

  • Akashi, T.; Aoki, T.; Ayabe, S.
    Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II (1998), FEBS Lett., 431, 287-290.
    View publication on PubMed
Search for more literature for 1.14.14.162

Cloned(Commentary)

Cloned (Comment) Organism
CYP Ge-5/CYP93B1, expression in Sf9 insect cells and in Saccharomyces cerevisiae BJ2168 Glycyrrhiza echinata

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome recombinant enzyme, expressed in Sf9 insect cells or yeast cells Glycyrrhiza echinata
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycyrrhiza echinata flavone biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Glycyrrhiza echinata P93149
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-

Reaction

Reaction Comment Organism Reaction ID
a flavanone + [reduced NADPH-hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O mechanism, stereoselectivity Glycyrrhiza echinata
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of liquiritigenin at C-2 and formation of licodione as a result of non-enzymatic hemiacetal opening, selectivity towards (2S)-flavanone Glycyrrhiza echinata licodione + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2 selectivity towards (2S)-flavanone Glycyrrhiza echinata 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O the product is further converted into apigenin when treated with HCl ?
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycyrrhiza echinata luteolin + [oxidized NADPH-hemoprotein reductase] + H2O product is formed after acid treatment ?
additional information flavone biosynthesis Glycyrrhiza echinata ?
-
 ?

Synonyms

Synonyms Comment Organism
(2S)-flavanone 2-hydroxylase
-
 Glycyrrhiza echinata
F2H
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 Glycyrrhiza echinata
LS
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 Glycyrrhiza echinata
More CYP93B1 encodes (2S)-flavanone 2-hydroxylase, which has previously been designated to licodione synthase and flavone synthase II Glycyrrhiza echinata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Glycyrrhiza echinata

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Glycyrrhiza echinata