Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in yeast microsomes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V359A | site-directed mutagenesis, the mutant shows altered product formation compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes with substrates 21-[2H]-progesterone, 21,21,21-[2H3]-progesterone, and 21,21,21-[2H3]-17-hydroxyprogesterone, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from yeast microsomes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | human CYP21A2 also has progesterone 16alpha-hydroxylase activity. Product analysis by LC-MS/MS | Homo sapiens | ? | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | mutant V359A produces an additional metabolite, 16alpha-hydroxyprogesterone of progesterone, very low activity with the wild-type enzyme | Homo sapiens | 16alpha-hydroxy-progesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product identification by TLC | ? |
Synonyms | Comment | Organism |
---|---|---|
P450c21 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |