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Literature summary for 1.14.14.16 extracted from

  • Ryan, K.J.; Engel, L.L.
    Hydroxylation of steroids at carbon 21 (1957), J. Biol. Chem., 225, 103-114.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
antimycin A 15% inhibition at 1mg per l Bos taurus
ascorbate 10% inhibition at 10 mM Bos taurus
azide 9% inhibition at 1 mM Bos taurus
carbon monoxide dark 65% inhibition at 90%, light 57% inhibition at 90% Bos taurus
CuSO4 50% inhibition at 1 mM Bos taurus
cyanide 14% inhibition at 1 mM Bos taurus
cytochrome c complete inhibition at 0.1 mM, 0.003 mM Bos taurus
diethylaminoethyldiphenylpropylacetic acid SKF 525 A 9% inhibition at 1 mM Bos taurus
HgCl2 complete inhibition at 0.1 mM Bos taurus
additional information not inhibited by catalase; not inhibited by iodoacetate, o-iodosobenzoate, glutathione, EDTA, dipyridyl, quinacrine, ribonuclease, cytochrome c + CN-; not inhibited by metal ions such as Cr3+, Fe3+, Zn2+, Pb2+, Mn2+, Co2+ at 1 mM Bos taurus
p-chloromercuribenzoate complete inhibition at 1 mM, but not inhibitory at 0.1 mM Bos taurus
sodium o-(3-hydroxymercuri-2-methoxypropyl)carbamyl-phenoxyacetc acid (mersalyl) complete inhibition at 1 mM Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Bos taurus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a steroid + electron donor + O2 Bos taurus essential step in synthesis of steroid hormones by adrenal gland a 21-hydroxysteroid + oxidized electron donor + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
beef
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11,17-dihydroxyprogesterone + NADH + O2
-
Bos taurus cortisol + NAD+ + H2O
-
?
11beta-hydroxyprogesterone + NADH + O2
-
Bos taurus corticosterone + NAD+ + H2O
-
?
17alpha-hydroxyprogesterone + NADH + O2
-
Bos taurus 17,21-dihydroxyprogesterone + NAD+ + H2O
-
?
a steroid + electron donor + O2 essential step in synthesis of steroid hormones by adrenal gland Bos taurus a 21-hydroxysteroid + oxidized electron donor + H2O
-
?
DELTA5-pregnen-3beta,17alpha-diol-20-one + NADH + O2
-
Bos taurus ? + NAD+ + H2O
-
?
DELTA5-pregnen-3beta-ol-20-one + NADH + O2
-
Bos taurus deoxycorticosterone + NAD+ + H2O very small yields, presumably via dehydrogenation followed by C-21 hydroxylation ?
progesterone + NADH + O2
-
Bos taurus deoxycorticosterone + NAD+ + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 about 50% of activity maximum at pH 5.5 and 8.0 Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADH NADH is 50% as effective as NADPH Bos taurus
NADPH
-
Bos taurus