Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antimycin A | 15% inhibition at 1mg per l | Bos taurus | |
ascorbate | 10% inhibition at 10 mM | Bos taurus | |
azide | 9% inhibition at 1 mM | Bos taurus | |
carbon monoxide | dark 65% inhibition at 90%, light 57% inhibition at 90% | Bos taurus | |
CuSO4 | 50% inhibition at 1 mM | Bos taurus | |
cyanide | 14% inhibition at 1 mM | Bos taurus | |
cytochrome c | complete inhibition at 0.1 mM, 0.003 mM | Bos taurus | |
diethylaminoethyldiphenylpropylacetic acid SKF 525 A | 9% inhibition at 1 mM | Bos taurus | |
HgCl2 | complete inhibition at 0.1 mM | Bos taurus | |
additional information | not inhibited by catalase; not inhibited by iodoacetate, o-iodosobenzoate, glutathione, EDTA, dipyridyl, quinacrine, ribonuclease, cytochrome c + CN-; not inhibited by metal ions such as Cr3+, Fe3+, Zn2+, Pb2+, Mn2+, Co2+ at 1 mM | Bos taurus | |
p-chloromercuribenzoate | complete inhibition at 1 mM, but not inhibitory at 0.1 mM | Bos taurus | |
sodium o-(3-hydroxymercuri-2-methoxypropyl)carbamyl-phenoxyacetc acid (mersalyl) | complete inhibition at 1 mM | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a steroid + electron donor + O2 | Bos taurus | essential step in synthesis of steroid hormones by adrenal gland | a 21-hydroxysteroid + oxidized electron donor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
beef | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11,17-dihydroxyprogesterone + NADH + O2 | - |
Bos taurus | cortisol + NAD+ + H2O | - |
? | |
11beta-hydroxyprogesterone + NADH + O2 | - |
Bos taurus | corticosterone + NAD+ + H2O | - |
? | |
17alpha-hydroxyprogesterone + NADH + O2 | - |
Bos taurus | 17,21-dihydroxyprogesterone + NAD+ + H2O | - |
? | |
a steroid + electron donor + O2 | essential step in synthesis of steroid hormones by adrenal gland | Bos taurus | a 21-hydroxysteroid + oxidized electron donor + H2O | - |
? | |
DELTA5-pregnen-3beta,17alpha-diol-20-one + NADH + O2 | - |
Bos taurus | ? + NAD+ + H2O | - |
? | |
DELTA5-pregnen-3beta-ol-20-one + NADH + O2 | - |
Bos taurus | deoxycorticosterone + NAD+ + H2O | very small yields, presumably via dehydrogenation followed by C-21 hydroxylation | ? | |
progesterone + NADH + O2 | - |
Bos taurus | deoxycorticosterone + NAD+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | about 50% of activity maximum at pH 5.5 and 8.0 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH is 50% as effective as NADPH | Bos taurus | |
NADPH | - |
Bos taurus |